Biodurability of Dentin-Adhesive Resin Bond Joint

牙本质粘合树脂粘接接头的生物耐久性

• 批准号: 6611396
• 负责人: STEVEN Ray ARMSTRONG
• 金额: $ 7.38万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6611396
• 关键词: adhesions; biomaterial interface interaction; biotransformation; clinical research; composite resins; dental adhesive /sealant; dental bonding material; dental caries; dental research; dentin; human tissue; inflammation; mass spectrometry; scanning electron microscopy; surface coating; tooth enamel; tooth root; tooth surface

DESCRIPTION (provided by applicant): A more reliable, technique-insensitive and durable adhesive resin bond to dentin is required if the full benefit of bonded restorations is to be realized. This is especially important due to the fact that caries remains a major health problem, dentin sensitivity affects greater than 40 million Americans, and root caries is increasing with our aging population. Adhesive bonding to enamel has proven to be highly successful; however, bonding to dentin remains poorly understood and unreliable. Resin adhesion to dentin is thought to be primarily micromechanical by the interdiffusion of adhesive monomers into the acid demineralized organic matrix; through steps not dissimilar to any other adhesive procedure, i.e. surface preparation, wetting and solidification. The long-range goal of our program of research is the conservative restoration of teeth, prevention of demineralization, and desensitization of exposed dentin surfaces through dentin bonding. The specific objective of this application is to identify resin-dentin bond degradation involved with the wetting process. The central hypothesis of this work is that the mineral depleted dentin surface must be completely and homogeneously infiltrated by the adhesive system for a successful dentin bond. Preliminary mechanical, fractographic and analytical work provide evidence that current dentin adhesive systems incompletely infiltrate the dentin surface, phase separate, polymerize sub-optimally, and hydrolytically degrade, leading to clinical restorative failure. The rationale for the proposed research is that, once mechanisms of dentin bond formation and failures are understood, evidence-based novel approaches for successful dentin adhesion can be developed. Aim 1 will determine probability failure rates and debond pathways of the dentin-resin composite bond exposed to simulated bacterial and tissue inflammatory responses utilizing a novel accelerated aging model. Aim 2 will identify the resin monomer degradation products from the storage media from Aim 1. Aim 3 will identify resin infiltration and stability throughout the hybrid layer using micro-Raman spectroscopy. We expect to demonstrate a decrease in bond strength, a changing debond pathway and a decreasing amount of adhesive monomer in the hybrid layer. This study is significant, because the knowledge gained will improve the understanding of dentin adhesion and biodurability; leading to hypothesis-driven clinical trials examining the wetting conditions required for a clinically successful dentin bond.

描述（由申请人提供）： 如果要充分发挥粘接牙本质的优势，就需要一种更可靠、技术不敏感和持久的粘接树脂。这一点尤其重要，因为龋齿仍然是一个主要的健康问题，牙本质敏感性影响超过4000万美国人，根龋随着人口老龄化而增加。牙釉质的粘接已被证明是非常成功的;然而，牙本质的粘接仍然知之甚少，也不可靠。树脂对牙本质的粘附被认为主要是通过粘合剂单体相互扩散到酸脱矿有机基质中的微观机械;通过与任何其他粘合剂程序相似的步骤，即表面准备、润湿和固化。我们的研究计划的长期目标是牙齿的保守修复，预防脱矿，并通过牙本质粘结对暴露的牙本质表面进行脱敏。本申请的具体目的是确定润湿过程中涉及的树脂-牙本质粘结降解。这项工作的中心假设是，矿物质耗尽的牙本质表面必须完全和均匀地渗透的粘合剂系统，以成功的牙本质粘结。初步的机械、断口分析和分析工作提供了证据，表明目前的牙本质粘合剂系统不完全渗入牙本质表面、相分离、粘附次优和水解降解，导致临床修复失败。这项研究的基本原理是，一旦了解了牙本质粘结形成和失效的机制，就可以开发出基于证据的成功牙本质粘附的新方法。 目的1将确定使用一种新的加速老化模型暴露于模拟细菌和组织炎症反应的牙本质-树脂复合粘结剂的失效率和脱粘途径。目标2将鉴别目标1储存介质中的树脂单体降解产物。目标3将使用显微拉曼光谱来识别整个混合层中的树脂渗透和稳定性。我们希望证明粘合强度降低，脱粘途径改变和混合层中粘合剂单体的量减少。 这项研究意义重大，因为获得的知识将提高对牙本质粘附和生物耐久性的理解;导致假设驱动的临床试验，检查临床成功牙本质粘结所需的润湿条件。

项目成果

Apparent conversion of adhesive resin in the hybrid layer, Part II: In situ studies of the resin-dentin bond.

混合层中粘合树脂的表观转化，第二部分：树脂-牙本质粘合的原位研究。

• DOI: 10.1002/jbm.a.31954
• 发表时间: 2009
• 期刊: Journal of biomedical materials research. Part A
• 作者: Zou,Yuan;Jessop,JulieLP;Armstrong,StevenR
• 通讯作者: Armstrong,StevenR

Apparent conversion of adhesive resin in the hybrid layer, Part 1: Identification of an internal reference for Raman spectroscopy and the effects of water storage.

混合层中粘合树脂的表观转化，第 1 部分：拉曼光谱内参的识别和水储存的影响。

• DOI: 10.1002/jbm.a.31684
• 发表时间: 2008
• 期刊: Journal of biomedical materials research. Part A
• 作者: Zou,Yuan;Armstrong,StevenR;Jessop,JulieLP
• 通讯作者: Jessop,JulieLP

Quantitative analysis of adhesive resin in the hybrid layer using Raman spectroscopy.

使用拉曼光谱对混合层中的粘合剂树脂进行定量分析。

• DOI: 10.1002/jbm.a.32692
• 发表时间: 2010
• 期刊: Journal of biomedical materials research. Part A
• 作者: Zou,Yuan;Armstrong,StevenR;Jessop,JulieLP
• 通讯作者: Jessop,JulieLP

Effects of exogenous collagenase and cholesterol esterase on the durability of the resin-dentin bond.

外源胶原酶和胆固醇酯酶对树脂-牙本质键耐久性的影响。

• 发表时间: 2006
• 期刊: The journal of adhesive dentistry
• 作者: Armstrong,SteveR;Jessop,JulieLP;Vargas,MarcosA;Zou,Yuan;Qian,Fang;Campbell,JoshuaA;Pashley,DavidH
• 通讯作者: Pashley,DavidH

In vitro enzymatic biodegradation of adhesive resin in the hybrid layer.

• DOI: 10.1002/jbm.a.32689
• 发表时间: 2010-07
• 期刊: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
• 影响因子: 4.9
• 作者: Zou, Yuan;Jessop, Julie L. P.;Armstrong, Steven R.
• 通讯作者: Armstrong, Steven R.