Enamel matrix derivative regulated genes

描述：（由申请人提供）在牙齿发育过程中， 上皮根鞘分泌釉基质蛋白。纯化的 来自发育中猪的釉质基质产物（釉质基质衍生物，EMD） 牙齿已经可以在市场上买到，并且已经成功地用于 修复牙周韧带、牙骨质和牙槽骨的功能。的 EMD的再生能力已在临床和动物实验中得到证实 问题研究然而，其生物学机制尚不清楚。我们假设 EMD调节成骨细胞的一些主要基因表达 分化、生长和功能。该项目的目标是 鉴定EMD处理的前成骨细胞系中调控的基因 利用DNA微阵列技术对MC 3 T3-E1和MG 63成骨样细胞进行了研究。 将通过北方印迹分析确认鉴定的感兴趣的基因。 找到这两种细胞系中的共同基因变化将有助于我们找到 基因参与EMD与成骨细胞之间的相互作用。的 两种细胞系之间EMD调节基因变化的差异将有助于 我们了解为什么EMD对前成骨细胞有不同的影响， 成骨细胞样细胞。我们认为，在完成拟议的 研究，我们的研究结果将构成足够的数据，为未来的R 01赠款 应用于EMD调控基因的研究， 这些基因与成骨细胞的生长和功能有关。的结果 应用程序还将为识别可能 在EMD中活跃以调节所鉴定的基因。已鉴定的分子 EMD可以更有效、更方便地用于牙周治疗， 与分离的EMD蛋白混合物相比，

DESCRIPTION: (provided by applicant) During tooth development, cells of Hertwig's epithelial root sheath secrete enamel matrix proteins. A purified enamel matrix product (Enamel Matrix Derivative, EMD) from developing porcine teeth became commercially available and has been successfully employed to restore fully functional periodontal ligament, cementum and alveolar bone. The regenerative capacity of EMD has been demonstrated in clinical and animal studies. However the biological mechanisms are not known. We hypothesize that EMD regulates some primary gene expression, which regulate osteoblast differentiation, growth and function. The objective of this project is to identify the genes regulated in the EMD treated preosteoblastic cell line MC3T3-E1 and MG63 osteoblast-like cells using DNA microarray technology. Identified interesting genes will be confirmed by northern blot analysis. Finding the common gene changes in both cell lines will help us to find which genes are involved in the interaction between EMD and osteoblasts. The difference of EMD regulated gene changes between the two cell lines will help us to understand why EMD have different effects on preosteoblasts and osteoblast-like cells. We believe that, at the completion of the proposed research, our findings will constitute sufficient data for a future R01 grant application to investigate the EMD regulated genes and the relationship of these genes with osteoblast growth and function. The results of this application will also provide foundation for identifying what molecules might be active in EMD to regulate the identified genes. The identified molecules in the EMD could be used more efficiently and conveniently for periodontal regeneration than the isolated EMD protein mixture.