CORE--CELLULAR AND MOLECULAR MORPHOLOGY

核心——细胞和分子形态学

• 批准号: 6588777
• 负责人: ANITA C GILLIAM
• 金额: $ 4.59万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6588777
• 关键词: bioimaging /biomedical imaging; biomedical facility; electron microscopy; fluorescent dye /probe; histology; histopathology; human tissue; immunocytochemistry; immunoelectron microscopy; in situ hybridization; light microscopy; microscopy; morphology; nucleic acid probes; scanning electron microscopy; scanning transmission electron microscopy; sectioning; skin disorder; tissue /cell preparation; tissue resource /registry

The Cellular and Molecular Morphology Core (CMMC) has existed since the beginning of the SDRC at our institutions. It has been consistently a heavily utilized Core providing a diverse array of services to a large number of SDRC faculty and their associates. In the current proposal, the principal aim of the CMMC is to continue to provide state-of-the-art services and expertise to SDRC investigators using molecular probes for a wide variety of morphological and molecular biological studies in which microscopic analysis plays a central role. These services include providing (i) immunolocalization and in situ hybridization methods for morphological studies, (ii) embedding, sectioning, and staining facilities and services, (iii) a centralized resource center that is designed to foster interaction and collaboration, (iv) scanning and transmission electron microscopy and immunoelectron microscopy services, (v) confocal laser microscopy, (vi) laser capture microscopy, (vii) expert-guided hands-on training in a range of microscopic technologies, (viii) a facility to house and catalog our 1,000 specimen human tissue sample library; and (ix) extraction of nucleic acids from those banked tissue specimens to maximize SDRC faculty access to DNA and RNA from specific skin diseases. The goals of the core including providing instrumentation, technical expertise and hands-on training in using molecular probes in morphological studies. An additional goal is to foster exchange of technical information among SDRC members and to encourage the sharing of resources. Key features of this core facility are its flexibility in adapting to the evolving needs of SDRC faculty, and its comprehensive range of services designed to support the needs of both proposed and future SDRC-related morphological studies involving cellular and extracellular molecules. The SDRC faculty responsible for CMMC operations function as a well-integrated team that collectively offers state-of-the-art expertise in all aspects of core function, and provides access to highly trained personnel in very well equipped research laboratories.

细胞和分子形态学核心（CMMC）自SDRC成立以来一直存在于我们的机构。它一直是一个高度利用的核心，为大量的SDRC教师及其同事提供各种各样的服务。在目前的提案中，CMMC的主要目标是继续为SDRC研究人员提供最先进的服务和专业知识，这些研究人员使用分子探针进行各种形态学和分子生物学研究，其中显微镜分析发挥着核心作用。这些服务包括提供（i）用于形态学研究的免疫定位和原位杂交方法，（ii）包埋，切片和染色设施和服务，（iii）旨在促进互动和合作的集中资源中心，（iv）扫描和透射电子显微镜和免疫电子显微镜服务，（v）共聚焦激光显微镜，（vi）激光捕获显微镜，（vii）专家指导的一系列显微技术的实践培训，（viii）一个设施，以容纳和编目我们的1,000个标本人体组织样本库;和（ix）从这些库存组织标本中提取核酸，以最大限度地提高SDRC教师获得特定皮肤病的DNA和RNA。核心的目标包括提供仪器，技术专长和实践培训，在形态学研究中使用分子探针。另一个目标是促进SDRC成员之间的技术信息交流，并鼓励资源共享。该核心设施的主要特点是其适应SDRC教师不断变化的需求的灵活性，以及旨在支持拟议和未来涉及细胞和细胞外分子的SDRC相关形态学研究的需求的全面服务。负责CMMC运营的SDRC教师作为一个整合良好的团队，共同提供核心功能各个方面的最先进的专业知识，并在设备齐全的研究实验室中提供训练有素的人员。