Monoclonal antibodies for Lassa fever vaccine design and therapeutics

用于拉沙热疫苗设计和治疗的单克隆抗体

• 批准号: 2275015
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2275015
• 关键词: Monoclonal; antibodies; Lassa; fever; vaccine

Using new improved methods for plasma cell Ig-cloning and to investigate antigen-staining memory B cell enrichment to isolate novel monoclonal antibodies to Lassa fever. There will also be the opportunity to utilise phage display techniques as an alternative approach to antibody isolation. Whilst antibody cloning is a key element of the work, the majority of the project will be spent characterising the antibodies to include epitope mapping, development of replication-competent chimeric VSV isolates and their use in antibody escape.

采用改良的浆细胞Ig克隆和抗原染色记忆B细胞富集方法，分离抗拉沙热单克隆抗体。也将有机会利用噬菌体展示技术作为抗体分离的替代方法。虽然抗体克隆是这项工作的一个关键要素，但该项目的大部分时间将用于表征抗体，包括表位作图，开发可复制的嵌合VSV分离株及其在抗体逃逸中的应用。