Determinants of Rod and Cone Response Characteristics

杆和锥响应特性的决定因素

• 批准号: 6649367
• 负责人: JAMES Bryant HURLEY
• 金额: $ 37.4万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6649367
• 关键词: arrestins; cone cell; dark adaptation; electrophysiology; electroretinography; flow cytometry; genetically modified animals; high performance liquid chromatography; laboratory mouse; light adaptations; mass spectrometry; phosphorylation; polymerase chain reaction; protein structure function; rhodopsin; rhodopsin kinase; rod cell; transducin; visual photoreceptor; visual photosensitivity; visual phototransduction; visual pigments

DESCRIPTION (provided by applicant): Photoreceptors have several important physiological functions. They detect light but they also adapt to constant illumination and darkness. Phosphorylation of rhodopsin is a critical biochemical reaction that modulates the phototransduction cascade. In this proposal we outline methods to precisely characterize the contributions of rhodopsin phosphorylation to light and dark adaptation and to the kinetics of the photoresponse. Rhodopsin phosphorylation will be measured both in isolated intact mouse retinas and in vivo using high-resolution rapid quench and mass spectrometry methodology. The time course of rhodopsin phosphorylation will be established under a variety of physiologically important conditions. Physiological responses of rod photoreceptors will be established by quantitative electroretinography under conditions identical to those used for phosphorylation measurements. A variety of mouse strains will be examined in which genes encoding proteins that influence rhodopsin phosphorylation and regeneration are mutated. Retinoid metabolism will be monitored and it will also be compared with the kinetics of physiological responses to light and darkness. These studies will precisely define the contributions of phosphorylation and visual cycle reactions to light and dark adaptation and photoresponse kinetics in mammalian retinas.

描述（由申请人提供）：光感受器具有几个重要的 生理功能。它们能探测到光线， 照明和黑暗。视紫红质的磷酸化是 调节光传导级联的生化反应。在这 建议我们概述的方法，以准确描述的贡献， 视紫红质磷酸化对光和暗适应的影响以及对 光反应。视紫红质磷酸化将在分离的 完整小鼠视网膜和体内使用高分辨率快速淬灭和质量 光谱测定法视紫红质磷酸化的时间过程将是 在各种生理学上重要的条件下建立。 视杆细胞光感受器的生理反应将通过 定量视网膜电图，条件与用于 磷酸化测量。将对各种小鼠品系进行检查， 哪些基因编码影响视紫红质磷酸化的蛋白质， 再生是变异的。将监测类维生素A代谢， 也可以与对光的生理反应的动力学进行比较， 山中贼易这些研究将准确地确定 磷酸化和视觉周期反应的光和暗适应， 哺乳动物视网膜的光反应动力学。