KSHV (HHV8) KAPOSIN ONCOGENE

描述：人类疱疹病毒8型(HHV-8或KSHV)已与KS和 体腔淋巴瘤。调查员的实验室显示，一架60 病毒mRNA编码的氨基酸kaposin蛋白在ALL中表达 感染的各个阶段。Kaposin可以在体外转化大鼠-3细胞，并且 转化的细胞在裸鼠体内产生了肿瘤。因此，kaposin可能会发挥一种 Kaposin在转化中起关键作用，进一步研究Kaposin具有重要意义。四 提出了具体的目标。第一个是确定转型 通过产生突变的kaposin来构建结构域。第二种是产生卡波辛 用特异性单抗研究Kaposin蛋白在体外培养细胞中的表达 KS和肿瘤细胞。第三个目标是确定卡波辛的效果。 单独表达或与其他KSHV转化基因联合表达 各种内皮细胞和淋巴样细胞。最后一个目标是确定 注重与增长相互作用的转化机制 因子受体和钙调素依赖的磷酸化靶点 激活剂。这项研究将为进一步了解其发病机制提供新的信息。 HHV-8。

DESCRIPTION: Human herpesvirus-8 (HHV-8 or KSHV) has been linked to KS and body cavity-based lymphomas. The investigator's laboratory showed that a 60 amino acid kaposin protein encoded by a viral mRNA is expressed in all stages of infection. Kaposin can transform Rat-3 cells in vitro, and the transformed cells caused tumors in nude mice. Therefore, kaposin may play a key role in transformation, and further study of kaposin is important. Four specific aims are proposed. The first is to determine the transforming domains by generating mutant kaposins. The second is to generate kaposin specific monoclonal antibodies to study the expression of kaposin protein in KS and tumor cells. The third aim is to determine the effects of kaposin expression by itself or in combination with other KSHV transforming genes on various endothelial cells and lymphoid cells. The last aim is to determine the mechanism of transformation by focusing on its interaction with growth factor receptors and as a target for phosphorylation by calmodulin-dependent kinase. This study should provide new information on the pathogenesis of HHV-8.