Core--Biotechnology

核心--生物科技

• 批准号: 6820120
• 负责人: Thomas R Martin
• 金额: $ 40.59万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6820120
• 关键词: animal tissue; biomedical facility; biotechnology; gene expression; histology; human tissue; immunologic assay /test; microarray technology; proteomics

The major objective of the Biotechnology Core is to provide state-of-the-art methodology in histologic methods, gene expression, and protein analysis to support the clinical and basic projects of the SCCOR. Dr. Martin will provide overall direction for the Core and will be assisted by Charles Frevert, DVM, ScD, Scientific Director of the Pulmonary Research Laboratories at VA Puget Sound Medical Center. Core B brings together investigators with state-of-the-art experience in tissue analysis, microarray analysis of gene expression, protein chemistry and proteomics to support the SCCOR clinical and basic investigators. The Specific Aims of the Core are: 1. To provide histologic analysis of tissue specimens provided by the basic science projects (Projects 4,5,6). 2. To provide gene expression analysis for the clinical and basic projects (Projects 1, 3, 4, 5, 6) using the expertise and facilities of the University of Washington Microarray Facility, R. Bumgamer, PhD, Director. 3. To provide: a) multiplex protein analysis by immunoassay in the Core Laboratory; b) analysis of specific protein modifications in the Protein Analysis Core Facility, University of Washington Department of Medicine; c) complex analysis of proteins in clinical and laboratory samples using proteomics technology available at the Institute for Systems Biology, Seattle, WA.

生物技术核心的主要目标是提供最先进的组织学方法、基因表达和蛋白质分析方法，以支持SCCOR的临床和基础项目。Martin博士将为核心提供总体指导，并将由VA Puget Sound医学中心肺研究实验室科学主任Charles Frevert， DVM， ScD协助。Core B汇集了在组织分析、基因表达微阵列分析、蛋白质化学和蛋白质组学方面具有最先进经验的研究人员，以支持SCCOR临床和基础研究人员。核心的具体目标是：