Core--Biotechnology

生物技术核心的主要目标是提供最先进的组织学方法，基因表达和蛋白质分析方法，以支持SCCOR的临床和基础项目。Martin博士将为核心提供总体指导，并将由VA Puget Sound医疗中心肺部研究实验室的科学总监Charles Frevert，DVM，ScD提供协助。核心B汇集了在组织分析、基因表达微阵列分析、蛋白质化学和蛋白质组学方面具有最先进经验的研究人员，以支持SCCOR临床和基础研究人员。核心的具体目标是： 1.对基础科学项目（项目4、5、6）提供的组织标本进行组织学分析。 2.利用华盛顿大学微阵列设施的专业知识和设备，为临床和基础项目（项目1，3，4，5，6）提供基因表达分析。Bumgamer，PhD，Director. 3.提供：B）在华盛顿大学的蛋白质分析核心设施中分析特异性蛋白质修饰 c）使用蛋白质分析仪对临床和实验室样品中的蛋白质进行复杂分析 蛋白质组学技术，可在华盛顿州西雅图的系统生物学研究所获得。

The major objective of the Biotechnology Core is to provide state-of-the-art methodology in histologic methods, gene expression, and protein analysis to support the clinical and basic projects of the SCCOR. Dr. Martin will provide overall direction for the Core and will be assisted by Charles Frevert, DVM, ScD, Scientific Director of the Pulmonary Research Laboratories at VA Puget Sound Medical Center. Core B brings together investigators with state-of-the-art experience in tissue analysis, microarray analysis of gene expression, protein chemistry and proteomics to support the SCCOR clinical and basic investigators. The Specific Aims of the Core are: 1. To provide histologic analysis of tissue specimens provided by the basic science projects (Projects 4,5,6). 2. To provide gene expression analysis for the clinical and basic projects (Projects 1, 3, 4, 5, 6) using the expertise and facilities of the University of Washington Microarray Facility, R. Bumgamer, PhD, Director. 3. To provide: a) multiplex protein analysis by immunoassay in the Core Laboratory; b) analysis of specific protein modifications in the Protein Analysis Core Facility, University of Washington Department of Medicine; c) complex analysis of proteins in clinical and laboratory samples using proteomics technology available at the Institute for Systems Biology, Seattle, WA.