CORE-- BEHAVIOR AND INFORMATICS

动物行为学和信息学核心将为专门研究中心(SCOR)的动物基础科学项目提供支持。该核心将具有以下具体职能： A.支持动物项目中协议行为的研究。这将涉及对休息/活动的研究，数据的计算机处理，并确保被研究的动物是正常的，而不是行为异常者。 核心工作人员将协助个别项目的工作人员进行睡眠(休息)剥夺研究和牺牲动物。 对于通过脑电(EEG)和肌电(EMG)方法记录睡眠/觉醒的研究，核心工作人员将在这项活动中协助项目人员，并负责对所有EEG/EMG记录进行手工评分。 B.使用鼠标信息学系统向SCOR的调查人员提供数据库支持，使调查人员能够存档和检索关于老鼠行为、动物健康细节、牺牲方法、组织解剖以及通过显微解剖技术获得的RNA或蛋白质数量的信息。 C.继续发展老鼠信息学中心，并开发一个研究果蝇的信息学中心。 D.通过大样本C57BL/6J小鼠的脑电/肌电记录来表征睡眠/觉醒模式，包括对不同睡眠剥夺时间的反应。 E.继续参与改进的非侵入性小鼠睡眠表型方法的开发。这将包括记录休息/活动和分析数字视频图像，并进行验证研究，将获得的数据与EEG/EMG方法确定的睡眠评分进行比较。

The Animal Behavioral and Informatics Core will provide support to the basic science animal projects in the Specialized Center of Research (SCOR). The core will have the following specific functions: a. To support studies of behavior for the protocols in the animal projects. This will involve studies of rest/activity, computer processing of data and ensuring that the animal being studied is normal and not a behavioral outlier. The Core staff will assist staff in the individual projects in conducting sleep (rest) deprivation studies and in sacrifice of animals. For studies involving sleep/wake recording by electroencephalographic (EEG) and electromyographic (EMG) means, the Core staffwill assist project staff in this activity and be responsible for hand scoring of all EEG/EMG records. b. To provide data base support to investigators in the SCOR using the Mouse Informatics system to allow investigators to archive and retrieve information on mouse behavior, details of animal health, method of sacrifice, tissue dissection and amount of RNA or protein obtained from microdissection techniques. c. To continue further development of the Mouse Informatics Center, and developing an Informatics Center for studies in Drosophila. d. To characterize sleep/wake patterns by EEG/EMG recording in a large sample of C57BL/6J mice during the behavioral protocols to be conducted, including the response to different durations of sleep deprivation. e. To continue participation in development of improved non-invasive ways of phenotyping mouse sleep. This will involve recording of rest/activity and analysis of digital video images with validation studies comparing the data obtained with definitive scoring of sleep by EEG/EMG methods.