CORE--TRANSGENIC MOUSE

(改编自申请人的摘要)本SCOR提案的鼠标核心 建立在四个现有核心或服务的基础上并与之集成 新英格兰医学中心分子心脏病学研究所和 塔夫茨大学医学院。这四个组成部分包括：1) MCRI转基因小鼠设施2)小鼠组织学服务，3) MCRI小鼠生理核心和4)MCRI细胞培养设施。《鼠标》 CORE服务于本SCOR的S 3、4、5项目，并与其紧密结合 关于实验相互作用的建议和细节可以在这些中找到 3个项目。四个MCRI组件中每个组件的描述 斯科尔鼠标核心是建立跟随。 MCRI转基因机构(Janis Lem，博士，主任)：MCRI转基因 设施位于新建的2500平方英尺的面积内， 通过原核产生两种标准转基因小鼠的能力 显微注射和胚泡注射基因敲除/敲除小鼠。包含 设施内有专门的动物舍，一个显微注射实验室，一个 分子生物学实验室，和一个组织培养室，以及一个冷藏室， 会议室和办公空间。这个动物室可容纳500人。 最先进的自我通风笼子(3,500只老鼠)，可容纳种马和 输精管结扎雄性小鼠和供受体雌性小鼠在生产中的应用 转基因和基因敲除/敲除小鼠。DVM玛丽·埃伦伯格博士负责监督 照顾和饲养动物，完全符合美国国立卫生研究院和 制度性指导方针。调查机构由以下机构认可 AAALAC，并拥有目前的PHS动物福利保险。

(Adapted from the Applicant's Abstract) The Mouse Core for this SCOR proposal is built on and integrated with four existing Cores or Services of the Molecular Cardiology Research Institute at New England Medical Center and Tufts University School of Medicine. These four components include: 1) the MCRI Transgenic Mouse Facility 2) The Mouse Tissue Histology Service, 3) the MCRI Mouse Physiology Core and 4) the MCRI Cell Culture Facility. The Mouse Core serves and is closely integrated with Project s 3, 4 and 5 of this SCOR proposal and details regarding experimental interactions may be found in these 3 projects. Descriptions of each of the four MCRI components upon which the SCOR Mouse Core is built follow. MCRI Transgenic Facility (Janis Lem, Ph.D., Director): The MCRI Transgenic facility is housed in a newly constructed 2500 square foot that houses the capabilities to produce both standard transgenic mice by pronuclear microinjection and knockout/knockin mice by blastocyst injection. Contained within the facility is dedicated animal housing, a microinjection lab, a molecular biology lab, and a tissue culture room, along with a cold room, conference room, and office space. The animal room has a capacity of -500 state-of-the-art self-ventilated cages (3,500 mice) to house stud and vasectomized males and donor and recipient female mice used in the production of transgenic and knockout/knockin mice. Dr. Mary Ellenberger, DVM, oversees the care and maintenance of animals, fully in accordance with NIH and institutional guidelines. The investigators Institution is accredited by AAALAC and has current PHS Animal Welfare Assurance.