CORE--TRANSGENIC MOUSE

（摘自申请人摘要）本SCOR提案的鼠标核心 是建立在四个现有的核心或服务，并与之集成， 新英格兰医学中心的分子心脏病学研究所和 塔夫茨大学医学院。这四个组成部分包括： MCRI转基因小鼠设施2）小鼠组织组织学服务，3） MCRI小鼠生理学核心和4）MCRI细胞培养设施。鼠标 核心服务与本SCOR的项目3、4和5紧密结合 关于实验相互作用的建议和细节可以在这些文件中找到。 3个项目。四个MCRI组件的描述， SCOR鼠标核心是建立如下。 MCRI转基因设施（Janis Lem，Ph.D.，导演：The MCRI Transgenic 设施位于新建的2500平方英尺， 通过原核技术生产标准转基因小鼠的能力 显微注射和通过胚泡注射的敲除/敲入小鼠。载 该设施内有专门的动物房，显微注射实验室， 分子生物学实验室，和一个组织培养室，沿着一个冷藏室， 会议室和办公室。动物房可容纳-500人 最先进的自通风笼（3，500只小鼠），用于饲养种马， 生产中使用的输精管切除雄性和供体及受体雌性小鼠 转基因和基因敲除/基因敲入小鼠。玛丽埃伦伯格博士，DVM，监督 动物的护理和维护，完全按照NIH和 体制指导方针。研究机构由 AAALAC，并具有当前的PHS动物福利保证。

(Adapted from the Applicant's Abstract) The Mouse Core for this SCOR proposal is built on and integrated with four existing Cores or Services of the Molecular Cardiology Research Institute at New England Medical Center and Tufts University School of Medicine. These four components include: 1) the MCRI Transgenic Mouse Facility 2) The Mouse Tissue Histology Service, 3) the MCRI Mouse Physiology Core and 4) the MCRI Cell Culture Facility. The Mouse Core serves and is closely integrated with Project s 3, 4 and 5 of this SCOR proposal and details regarding experimental interactions may be found in these 3 projects. Descriptions of each of the four MCRI components upon which the SCOR Mouse Core is built follow. MCRI Transgenic Facility (Janis Lem, Ph.D., Director): The MCRI Transgenic facility is housed in a newly constructed 2500 square foot that houses the capabilities to produce both standard transgenic mice by pronuclear microinjection and knockout/knockin mice by blastocyst injection. Contained within the facility is dedicated animal housing, a microinjection lab, a molecular biology lab, and a tissue culture room, along with a cold room, conference room, and office space. The animal room has a capacity of -500 state-of-the-art self-ventilated cages (3,500 mice) to house stud and vasectomized males and donor and recipient female mice used in the production of transgenic and knockout/knockin mice. Dr. Mary Ellenberger, DVM, oversees the care and maintenance of animals, fully in accordance with NIH and institutional guidelines. The investigators Institution is accredited by AAALAC and has current PHS Animal Welfare Assurance.