Fluorescence System for Sensing Biospecific Interactions

描述（由申请人提供）：一种创新的荧光 用于检测生物分子相互作用的系统是在第一阶段开发的， 基于全内反射荧光（TIRF）。主要 该系统的优点是集成的微流体和 允许快速更换TIRF传感器芯片。建立一个 成功研制了一种高灵敏度、多功能的TIRF生物传感器 在第一阶段展示。在第二阶段，我们将设计和建造一个完整的 交钥匙TIRF仪器。拟议的仪器将配备 集成射流技术，自动化样品处理模块和精密 TIRF传感器芯片的快速更换机制。各种 廉价的轮胎传感器芯片封装在墨盒将可用于 方便地固定不同的生物分子。通过这种方式， TIRF生物传感器仪器将被设计用于常规调查， 生物分子相互作用在第二阶段的第一年，我们建议建立 五个单位的TIRF生物传感器仪器。第二年，TIRF 仪器会在顾问公司的实验室和我们的 实验室在第二阶段结束时，一个经过微调的工业原型将被 得到了TIRF生物传感器仪器将于2010年建造和测试。 与C&L Instruments和我们的顾问密切合作。 的TIRF 生物传感器仪器是一种非常需要的工具，用于与结构相关的研究。 基因组学，蛋白质组学和其他领域的详细知识， 生物分子相互作用是重要的。由于拟议的文书将 将困难的TIRF实验转化为常规测量， 生物传感器有可能在许多领域彻底改变研究， 包括癌症研究、生物材料研究、药物发现、细胞 信号、基因组学和蛋白质组学、纳米技术、免疫测定和疫苗 发展

DESCRIPTION (provided by applicant): An innovative fluorescence system for sensing biomolecular interactions was developed in Phase I that is based on total internal reflection fluorescence (TIRF). The main advantages of this system are integrated microfluidics and a design that allows rapid replacement of TIRF sensor chip. The feasibility of building a highly sensitive and versatile TIRF biosensor instrument was successfully demonstrated in Phase I. In Phase II, we will design and build a complete turn-key TIRF instrument. The proposed instrument will be equipped with integrated fluidics, an automated sample handling module and a precision mechanism for rapid replacement of TIRF sensor chips. A variety of inexpensive TIRE sensor chips enclosed in cartridges will be available for convenient immobilization of different biomolecules. In this manner, the TIRF biosensor instrument will be designed for routine investigation of biomolecular interactions. During year one of Phase II, we propose to build five units of the TIRF biosensor instrument. In year two, the TIRF instrument will be tested in laboratories of our consultants and in our own lab. At the end of Phase II a fine-tuned industrial prototype wilt be obtained. The TIRF biosensor instrument will be constructed and tested in close collaboration with C&L Instruments and our consultants. A TIRF biosensor instrument is a much-needed tool for studies related to structural genomics, proteomics and other fields where detailed knowledge of biomolecular interactions are important. Since the proposed instrument will transform difficult TIRF experiments into routine measurements, the TIRF biosensor has the potential to revolutionize studies in many fields, including cancer research, biomaterials studies, drug discovery, cell signaling, genomics and proteomics, nanotechnology, immunoassay and vaccine development.