Fluorescence System for Sensing Biospecific Interactions

用于传感生物特异性相互作用的荧光系统

• 批准号: 6552037
• 负责人: ALEXANDER N ASANOV
• 金额: $ 64.25万
• 依托单位: BIOELECTROSPEC, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6552037
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; biosensor device; electrochemistry; fluorescence; fluorescence spectrometry; functional /structural genomics; intermolecular interaction; protein protein interaction; proteomics

DESCRIPTION (provided by applicant): An innovative fluorescence system for sensing biomolecular interactions was developed in Phase I that is based on total internal reflection fluorescence (TIRF). The main advantages of this system are integrated microfluidics and a design that allows rapid replacement of TIRF sensor chip. The feasibility of building a highly sensitive and versatile TIRF biosensor instrument was successfully demonstrated in Phase I. In Phase II, we will design and build a complete turn-key TIRF instrument. The proposed instrument will be equipped with integrated fluidics, an automated sample handling module and a precision mechanism for rapid replacement of TIRF sensor chips. A variety of inexpensive TIRE sensor chips enclosed in cartridges will be available for convenient immobilization of different biomolecules. In this manner, the TIRF biosensor instrument will be designed for routine investigation of biomolecular interactions. During year one of Phase II, we propose to build five units of the TIRF biosensor instrument. In year two, the TIRF instrument will be tested in laboratories of our consultants and in our own lab. At the end of Phase II a fine-tuned industrial prototype wilt be obtained. The TIRF biosensor instrument will be constructed and tested in close collaboration with C&L Instruments and our consultants. A TIRF biosensor instrument is a much-needed tool for studies related to structural genomics, proteomics and other fields where detailed knowledge of biomolecular interactions are important. Since the proposed instrument will transform difficult TIRF experiments into routine measurements, the TIRF biosensor has the potential to revolutionize studies in many fields, including cancer research, biomaterials studies, drug discovery, cell signaling, genomics and proteomics, nanotechnology, immunoassay and vaccine development.

描述(申请人提供)：一种创新的荧光 在第一阶段开发了生物分子相互作用传感系统， 是基于全内反射荧光(TIRF)。主 该系统的优点是集成了微流体和一种 允许快速更换TIRF传感器芯片。建造一座 研制成功高灵敏度、多用途的TIRF生物传感器 在第一阶段中演示。在第二阶段中，我们将设计和建造完整的 交钥匙TIRF仪器。拟议的文书将配备 集成的流体、自动样品处理模块和高精度 快速更换TIRF传感器芯片的机制。各种各样的 盒装的廉价轮胎传感器芯片将用于 方便地固定化不同的生物分子。通过这种方式， TIRF生物传感器仪器将被设计用于常规研究 生物分子相互作用。在第二阶段的第一年，我们建议建造 TIRF生物传感器仪器的五个单元。在第二年，TIRF 仪器将在我们顾问的实验室和我们自己的实验室进行测试 实验室。在第二阶段结束时，将有一个微调的工业原型 获得。TIRF生物传感器仪器将在#年建造和测试 与C&L仪器和我们的顾问密切合作。一个TIRF 生物传感仪器是结构研究中急需的工具。 基因组学、蛋白质组学和其他领域的详细知识 生物分子间的相互作用很重要。由于拟议的文书将 将困难的TIRF实验转化为常规测量 生物传感器有可能给许多领域的研究带来革命性的变化， 包括癌症研究、生物材料研究、药物发现、细胞 信号传递、基因组学和蛋白质组学、纳米技术、免疫分析和疫苗 发展。