Quantitation Of Viral Reproduction

目前还没有可靠的通用方法来量化病毒平均繁殖的时间。从宏观的角度来看，培养物中“平均”病毒的产仔数和繁殖时间是多少？该项目发展数学和实验技术，以确定单个母病毒粒子的子病毒粒子的平均数量，这些子病毒粒子附着在细胞上的时间，子病毒粒子的平均周期时间，以及病毒周期时间的标准偏差（传播）。这些测定可以通过流式细胞术实验进行。靶细胞用脂质染料染色，病毒与染色的“第0代”细胞孵育，在其附着期同步。通过收集感染标志物的数据（在实际实验中，这将是艾滋病毒的gp41），在第0代染色细胞和未染色细胞中，随后加入并与感染的“第0代”细胞孵育，平均病毒的繁殖可以确定为积分方程的解。原则上，同样的方法可以用于计算动物的产仔数和繁殖周期时间，在一组同类动物被释放到野外并进行几次种群计数之后。然而，在医学方面，这项工作旨在改善各种艾滋病毒疗法的定量及其对艾滋病毒繁殖的抑制作用。计算机程序现在已经就位，一篇理论论文已经发表，实验方案已经开始实施，数据目前正在分析中。J. L. Spouge和S. P. Layne“同时确定病毒的有效爆发大小和周期时间的实用方法”（1999年）《自然科学进展》。学会科学。美国96:7017-7022

There is no dependable general way of quantifying the times at which an average virus reproduces. In macroscopic terms, what are the litter sizes and breeding times of an "average" virus in a culture? This project develops mathematical and experimental techniques for determining the average number of daughter virions from a single mother virion, when these daughters attach to their cell, the average cycle time for the daughters, and the standard deviation (spread) of the viral cycle time. These determinations can be made from flow cytometry experiments. Target cells are stained with lipid dyes, and viruses syncronized at their attachment phase by incubating them with the stained "generation 0" cells. By collecting data on a marker of infection (in actual experiments, this will be gp41 in HIV), both in generation 0 stained cells and unstained cells that are added later and incubated with the infected "generation 0" cells, the reproduction of the average virus can be determined as the solution of an integral equation. The same methods can in principle be employed in calculating litter sizes and breeding cycle times of an animal, after a homogeneous group of the animals is released into the wild and several populaton counts taken. In the medical context, however, this work is intended to improve the quantitation of various HIV therapies and their inhibitory effects on HIV reproduction. Computer programs are now in place, a theoretical paper has been published, experimental protocols have now been carried out, and the data are currently being analyzed. J. L. Spouge and S. P. Layne "A practical method for simultaneously determining the effective burst sizes and cycle times of viruses" (1999) Proc. Natl. Acad. Sci. USA 96 : 7017-7022