Optical Dosimetry in Laser-Assisted Cancer Immunotherapy

描述(由申请人提供)：这项工作涉及激光辅助免疫疗法的光学剂量学方面，这是一种用于癌症治疗的新治疗方式。这种疗法在激光照射之前，将一种光吸收染料和一种免疫佐剂联合应用到肿瘤体积中。转移性乳腺肿瘤细胞将被植入Balb/C小鼠体内。在红外线吸收染料和免疫佐剂联合给药后，将用半导体激光照射肿瘤。这个项目的长期目标是确定能够激发强烈的肿瘤反应的光剂量，以完全根除原发肿瘤和远处转移。激光照射将启动对肿瘤的完全光热破坏，导致组织坏死。肿瘤的坏死程度是肿瘤温度的一个重要因素，它应该通过抗原提呈来决定肿瘤的反应。对于预测的肿瘤温度分布，将根据动态损伤模型获得标准生物热传递方程的数值解。光剂量将导致肿瘤持续而有力的免疫反应的最佳坏死，将通过实验确定。

DESCRIPTION (provided by applicant): This work is concerned with the optical dosimetric aspect of Laser-Assisted Immunotherapy, a novel treatment modality for cancer therapy. The therapy utilizes co-administration of a light absorbing dye and an immunoadjuvant into the tumor volume prior to laser irradiation. Metastatic mammary tumor cells will be implanted in Balb/C mice. The tumor will be irradiated with a diode laser following the co-administration of an infrared absorbing dye and an immunoadjuvant. The long-term objective of this project is to determine the light dose which would invoke a strong tumor response for the total eradication of the primary tumor and distant metastases. A complete photo-thermal destruction of the tumor will be initiated with the laser irradiation, resulting in tissue necrosis. The extent of necrosis, which should determine the tumor response through antigen presentation, is a strong function of the tumor temperature. Numerical solution of the standard bioheat transfer equation in the light of a dynamic damage model will be obtained for the predicted temperature profile of the tumor. The light dose, which would induce an optimum necrosis for a sustained and vigorous immune response of the tumor, will be experimentally determined.