Access to physical activity and its relation to health

获得身体活动及其与健康的关系

• 批准号: 6597147
• 负责人: HELAINE MARY ALESSIO
• 金额: $ 20.1万
• 依托单位: MIAMI UNIVERSITY OXFORD
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6597147
• 关键词: aging; animal morbidity; animal mortality; antioxidants; biomarker; blood lipid; blood pressure; body weight; cardiovascular disorder risk; cardiovascular function; cognition; exercise; heart rate; laboratory rat; longitudinal animal study; oxidative stress; postmortem; prolactin

DESCRIPTION (provided by applicant): Animal studies on mechanisms of oxidative stress, aging, and disease often include an experimental group receiving a certain intervention and a sedentary control group to which the experimental group is compared. The common use of a control group having no access to physical activity outside the cage poses a potential intervening variable that may affect results of studies investigating health and stress, in particular, oxidative stress. Animals participating in no physical activity outside of their cage may have different endogenous prooxidant and antioxidant levels, which over time may negatively affect their health. In previous studies where differences in biomarkers of oxidative stress or health were reported between control and experimental groups, investigators may have overestimated the effects of the intervention due to the hypokinetic effects of housing on the sedentary control animals. If animal studies are intended to generalize to other primate populations, including humans, then housing conditions and access to physical activity need to be carefully considered. The purpose of this study is to compare biomarkers of oxidative stress, a variety of health parameters, age and cause of death in animals that are grouped in three distinct ways: a) living solely in a standard cage with no access to physical activity outside the cage, 2) living in a standard cage with twice weekly one hour periods of physical activity in a large box, and 3) living in a standard cage with regular access to exercise on a running wheel. Cardiovascular parameters (blood pressure, heart rate, body weight, blood lipids) will be monitored weekly over the life span of the animals. Blood samples from a tail vein will be collected biannually and analyzed for antioxidants, lipids, and prolactin. Immediately after death, various tissues will be harvested and analyzed using a hematoxin and eosin staining method, and standard autopsy procedures performed by a veterinarian. Brain, ganglia, neurotrophic growth factor, pituitary gland, tumors, and organs and tissues will be analyzed for signs of pathology and differences among the three groups. Results of this study will distinguish cardiovascular parameters, morbidity, mortality, and biomarkers of oxidative stress due to different access to physical activity over the life span. These results will provide insight about the importance of housing and physical activity when designing animal experiments to study aging, selective health parameters, and oxidative stress.

描述（由申请人提供）：关于氧化应激、衰老和疾病机制的动物研究通常包括接受某种干预的实验组和与实验组进行比较的久坐对照组。通常使用无法在笼子外进行身体活动的对照组，这构成了一个潜在的干预变量，可能会影响健康和压力（特别是氧化应激）研究的结果。在笼外不参与任何体力活动的动物可能具有不同的内源性促氧化剂和抗氧化剂水平，随着时间的推移可能会对其健康产生负面影响。在先前的研究中，对照组和实验组之间报告了氧化应激或健康生物标志物的差异，研究人员可能高估了干预的效果，因为住房对久坐的对照动物的运动功能减退。如果动物研究是为了推广到其他灵长类动物种群，包括人类，那么住房条件和获得体力活动需要仔细考虑。本研究的目的是比较氧化应激的生物标志物，各种健康参数，动物的年龄和死亡原因，这些动物以三种不同的方式分组：a）仅生活在标准笼中，不能在笼外进行身体活动，2）生活在标准笼中，每周两次在大盒子中进行一小时的身体活动，和3）生活在一个标准的笼子里，有规律地在跑步轮上锻炼。在动物的生命周期内，每周监测一次心血管参数（血压、心率、体重、血脂）。每半年采集一次尾静脉血样，并分析抗氧化剂、脂质和催乳素。死亡后立即采集各种组织，并由兽医使用苏木精和伊红染色方法和标准尸检程序进行分析。将分析脑、神经节、神经营养生长因子、脑垂体、肿瘤以及器官和组织的病理学体征和三组间的差异。这项研究的结果将区分心血管参数，发病率，死亡率和氧化应激的生物标志物，由于不同的身体活动在整个生命周期。这些结果将提供有关住房和体育活动的重要性的见解时，设计动物实验，研究衰老，选择性健康参数和氧化应激。

项目成果

Revisiting influences on tumor development focusing on laboratory housing.

重新审视对肿瘤发展的影响，重点关注实验室住房。

• 发表时间: 2009
• 期刊: Journal of the American Association for Laboratory Animal Science : JAALAS
• 作者: Alessio,HelaineM;Schweitzer,NatalieB;Snedden,AngelaM;Callahan,Phyllis;Hagerman,AnnE
• 通讯作者: Hagerman,AnnE

Access to exercise and its relation to cardiovascular health and gene expression in laboratory animals.

实验动物的运动机会及其与心血管健康和基因表达的关系。

• DOI: 10.1016/j.lfs.2005.01.036
• 发表时间: 2005
• 期刊: Life sciences
• 影响因子: 6.1
• 作者: Schweitzer,NatalieB;Alessio,HelaineM;Hagerman,AnnE;Roy,Sashwati;Sen,ChandanK;Nagy,Szilvia;Byrnes,RobynN;Philip,BenN;Woodward,JaneL;Wiley,RonaldL
• 通讯作者: Wiley,RonaldL