INDIVIDUAL PREDOCTORAL DENTAL SCIENTIST FELLOWSHIP
个人博士前牙科科学家奖学金
基本信息
- 批准号:6634592
- 负责人:
- 金额:$ 3.72万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-03-10 至
- 项目状态:未结题
- 来源:
- 关键词:BCL2 gene /protein Retroviridae SCID mouse angiogenesis angiogenesis factor biodegradable product bone morphogenetic proteins capillary enzyme linked immunosorbent assay immunocytochemistry method development northern blottings osteoblasts osteogenesis polymers tissue engineering tissue support frame transfection /expression vector vascular endothelium western blottings
项目摘要
The field of tissue engineering has developed due to the inadequate supply of organs and tissues for patients requiring organ/tissue replacement. An attractive alternative to organ and tissue transplantation that could immensely expand clinicians' ability to treat patients requiring new organs and tissues is the selective transplantation of the appropriate cell type(s). Transplanting cell populations on biodegradable polymer matrices in order to engineer a functional tissue mass from these transplanted cells is an intriguing strategy. However, the survival of these cells is dependent on diffusion of nutrients and waste products between the transplanted cells on the matrix and the vasculature existing in the surrounding host tissue. Currently, this vascular network is the limiting factor in allowing the engineering of large-scale masses of tissue capable of forming whole organs. The proposed research focuses on strategies to increase the vascular network within these polymer matrices in order to create significant amounts of bone tissue capable of replacing osseous defects. The specific aims of this proposal are: Aim 1-To define strategies to engineer microvascular endothelial cells to produce growth factors/mediators that a) will induce a vigorous and sustained angiogenic response, and b) will induce bone development and growth from osteogenic precursors. We will initially look at the mediators Bcl-2, angiopoietin (two potent inducers of angiogenesis), and the osteogenic mediator BMP-2; Aim 2- To incorporate these different endothelial cell populations into biodegradable polymer matrices and to test their individual and combined abilities to form capillaries in vitro and in vivo; Aim 3- To determine the baseline bone formation when osteo-progenitor cells are transplanted on a biodegradable matrix; Aim 4-To determine if an appropriate combination of endothelial cells, osteo-progenitor cells, and matrix, as determined in Specific Aims 1-3, will result in a sustained and vigorous angiogenic response in vivo (SCID mouse model) capable of supporting bone development from osteo-progenitor cells.
由于需要器官/组织置换的患者的器官和组织供应不足,组织工程领域已经发展。 器官和组织移植的一个有吸引力的替代方案是选择性移植适当的细胞类型,这可以极大地扩展临床医生治疗需要新器官和组织的患者的能力。将细胞群移植到可生物降解的聚合物基质上,以便从这些移植的细胞中工程化功能性组织块是一种有趣的策略。 然而,这些细胞的存活依赖于基质上的移植细胞与周围宿主组织中存在的脉管系统之间的营养物和废物的扩散。目前,这种血管网络是允许工程化能够形成整个器官的大规模组织块的限制因素。 拟议的研究重点是增加这些聚合物基质内的血管网络的策略,以创造能够替代骨缺损的大量骨组织。 本提案的具体目的是:目的1-定义设计微血管内皮细胞以产生生长因子/介质的策略,所述生长因子/介质a)将诱导有力和持续的血管生成反应,和B)将诱导骨发育和从成骨前体生长。 我们将首先研究Bcl-2、血管生成素(两种有效的血管生成诱导剂)和成骨介质BMP-2;目的2-将这些不同的内皮细胞群掺入可生物降解的聚合物基质中,并测试它们在体外和体内形成毛细血管的单独和组合能力;目的3-确定当骨祖细胞移植到可生物降解基质上时的基线骨形成;目的4-确定内皮细胞、骨祖细胞和基质的适当组合,如在特定目的1-3中确定的,将导致体内持续而强烈的血管生成反应(SCID小鼠模型),能够支持骨祖细胞的骨发育。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
DARNELL KAIGLER其他文献
DARNELL KAIGLER的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('DARNELL KAIGLER', 18)}}的其他基金
Redefining mesenchymal stem cells: using their cellular and molecular phenotypes to determine their regenerative and therapeutic properties
重新定义间充质干细胞:利用其细胞和分子表型来确定其再生和治疗特性
- 批准号:
10468688 - 财政年份:2019
- 资助金额:
$ 3.72万 - 项目类别:
Redefining mesenchymal stem cells: using their cellular and molecular phenotypes to determine their regenerative and therapeutic properties
重新定义间充质干细胞:利用其细胞和分子表型来确定其再生和治疗特性
- 批准号:
10231011 - 财政年份:2019
- 资助金额:
$ 3.72万 - 项目类别:
Redefining mesenchymal stem cells: using their cellular and molecular phenotypes to determine their regenerative and therapeutic properties
重新定义间充质干细胞:利用其细胞和分子表型来确定其再生和治疗特性
- 批准号:
10687814 - 财政年份:2019
- 资助金额:
$ 3.72万 - 项目类别:
Customized craniofacial stem cell therapy for craniofacial bone defects
颅面骨缺损定制颅面干细胞治疗
- 批准号:
9135822 - 财政年份:2015
- 资助金额:
$ 3.72万 - 项目类别:
Customized craniofacial stem cell therapy for craniofacial bone defects
颅面骨缺损的定制颅面干细胞治疗
- 批准号:
9147559 - 财政年份:2015
- 资助金额:
$ 3.72万 - 项目类别:
Alveolar Bone Marrow Derived Stem Cells for Clinical Cell Therapy
用于临床细胞治疗的肺泡骨髓干细胞
- 批准号:
8733350 - 财政年份:2013
- 资助金额:
$ 3.72万 - 项目类别:
INDIVIDUAL PREDOCTORAL DENTAL SCIENTIST FELLOWSHIP
个人博士前牙科科学家奖学金
- 批准号:
6705025 - 财政年份:2002
- 资助金额:
$ 3.72万 - 项目类别:
INDIVIDUAL PREDOCTORAL DENTAL SCIENTIST FELLOWSHIP
个人博士前牙科科学家奖学金
- 批准号:
6516371 - 财政年份:2002
- 资助金额:
$ 3.72万 - 项目类别:
INDIVIDUAL PREDOCTORAL DENTAL SCIENTIST FELLOWSHIP
个人博士前牙科科学家奖学金
- 批准号:
6397736 - 财政年份:2001
- 资助金额:
$ 3.72万 - 项目类别:
INDIVIDUAL PREDOCTORAL DENTAL SCIENTIST FELLOWSHIP
个人博士前牙科科学家奖学金
- 批准号:
6135369 - 财政年份:2000
- 资助金额:
$ 3.72万 - 项目类别: