Phytochemical Treatments for Vocal Fold Scar

描述(申请人提供)：内源性声带疤痕导致严重的发音困难，这是很难治疗成功的。透明质酸(HA)是声带固有层细胞外基质中的一种重要分子，对组织粘度、声带振荡的启动和传播有重要作用。在伤口愈合过程中最大化术后HA水平的技术可能为优化组织粘弹性和最小化医源性瘢痕对声带生物力学特性的影响提供临床有用的方法。松果菊苷、花青苷和齐墩果酸是增加愈合伤口中HA沉积的植物化学物质。这些植物化学物质可能为治疗疤痕提供有用的治疗方法。这项建议旨在：1)定量声带瘢痕中HA的水平，2)评估植物化学处理对HA沉积的影响，以及3)定量处理和未处理组织样本的粘弹性剪切组织特性。组织学染色将用于检测HA，流变学方法将用于测量组织粘弹性。据推测，经处理的声带将显示更多的HA沉积，并在术后呈现较少的硬度和动态粘度，这意味着经植物化学处理的组织的粘弹性增强。

DESCRIPTION (provided by applicant): latrogenic vocal fold scarring results in a severe dysphonia, which is difficult to treat successfully. Hyaluronic acid (HA) is an important molecule found in the extracellular matrix of the vocal fold lamina propria, that contributes to tissue viscosity, and the initiation and propogation of vocal fold oscillation. Techniques aimed at maximizing post-operative HA levels during wound healing may offer clinically useful methods for optimizing tissue viscoelasticity, and minimizing the effects of iatrogenic scarring on vocal fold biomechanical properties. Echinacoside, Anthocyanoside, and Oleanolic Acid are phytochemicals that increase the deposition of HA in healing wounds. These phytochemicals may provide useful treatments for scarring. This proposal aims to: 1) Quantify HA levels in vocal fold scar, 2) Assess the effects of phytochemical treatments on deposition of HA, and 3) Quantify the viscoelastic shear tissue properties of treated and untreated tissue samples. Histologic staining will be used to assay HA, and rheologic methods will be used for measuring tissue viscoelasticity. It is hypothesized that the treated vocal fold will show greater deposition of HA, and present with less stiffness and dynamic viscosity post-operatively, signifying enhanced viscoelastic properties in phytochemically treated tissue.