Phytochemical Treatments for Vocal Fold Scar

描述（申请人提供）：内源性声带瘢痕导致严重的发声障碍，很难成功治疗。透明质酸（HA）是存在于声带固有层细胞外基质中的一种重要分子，它有助于组织黏性，以及声带振荡的发生和传播。旨在在伤口愈合过程中最大化术后HA水平的技术可能为优化组织粘弹性和最小化医源性瘢痕对声带生物力学特性的影响提供临床有用的方法。紫锥花苷、花青素苷和齐墩果酸是植物化学物质，可以增加愈合伤口中HA的沉积。这些植物化学物质可以有效地治疗疤痕。本研究旨在：1)量化声带瘢痕中的HA水平；2)评估植物化学处理对HA沉积的影响；3)量化处理和未处理组织样本的粘弹性剪切组织特性。组织学染色将用于测定透明质酸，流变学方法将用于测量组织粘弹性。据推测，治疗后的声带会显示更多的HA沉积，并且术后出现更少的刚度和动态粘度，这表明植物化学治疗组织的粘弹性增强。

DESCRIPTION (provided by applicant): latrogenic vocal fold scarring results in a severe dysphonia, which is difficult to treat successfully. Hyaluronic acid (HA) is an important molecule found in the extracellular matrix of the vocal fold lamina propria, that contributes to tissue viscosity, and the initiation and propogation of vocal fold oscillation. Techniques aimed at maximizing post-operative HA levels during wound healing may offer clinically useful methods for optimizing tissue viscoelasticity, and minimizing the effects of iatrogenic scarring on vocal fold biomechanical properties. Echinacoside, Anthocyanoside, and Oleanolic Acid are phytochemicals that increase the deposition of HA in healing wounds. These phytochemicals may provide useful treatments for scarring. This proposal aims to: 1) Quantify HA levels in vocal fold scar, 2) Assess the effects of phytochemical treatments on deposition of HA, and 3) Quantify the viscoelastic shear tissue properties of treated and untreated tissue samples. Histologic staining will be used to assay HA, and rheologic methods will be used for measuring tissue viscoelasticity. It is hypothesized that the treated vocal fold will show greater deposition of HA, and present with less stiffness and dynamic viscosity post-operatively, signifying enhanced viscoelastic properties in phytochemically treated tissue.