Minority Predoctoral Fellowship Program

少数族裔博士前奖学金计划

• 批准号: 6609728
• 负责人: NIKKI A DELK
• 金额: $ 3.62万
• 依托单位: RICE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-16 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6609728
• 关键词: Arabidopsis; biological signal transduction; calcium binding protein; calcium ion; calmodulin; gene expression; genetic manipulation; genetically modified plants; mutant; northern blottings; plant physiology; predoctoral investigator; protein binding; protein structure function; southern blotting; western blottings

DESCRIPTION (provided by applicant): Cells mount responses to extracellular stimuli by triggering changes in levels of second messengers, such as calcium, which then activate intracellular mediators of physiological response, such as calmodulin. Understanding how calmodulin-related proteins function will be valuable for elucidating signal transduction mechanisms in organisms.The purpose of this research is to determine the physiological and biochemical functions of the Arabidopsis calmodulin-related protein, TCH2. TCH2 mRNA accumulates in response to touch, wind, wounding, and darkness (Braam and Davis, 1990). TCH2 protein is 44% identical to calmodulin (Khan et al., 1997), suggesting a role for Ca2+ in TCH2 function. It is proposed that TCH2 is activated by Ca2+ and is involved in regulating a signaling pathway that translates external stimuli into a physiological response. To test this hypothesis, TCH2 mutants will be sought and characterized to determine the physiological function of TCH2. The biochemical role of TCH2 will be explored by screening for genes that encode proteins that bind TCH2 in a Ca2+-dependent manner. This research will provide insight as to how cells use Ca2+ to sense and respond to extracellular stimuli.

描述（由申请人提供）：细胞通过触发第二信使（如钙）水平的变化，对细胞外刺激产生反应，然后激活细胞内生理反应介质，如钙调素。了解钙调素相关蛋白的功能将有助于阐明生物体内的信号转导机制。本研究的目的是确定拟南芥钙调素相关蛋白TCH2的生理生化功能。TCH2 mRNA在触摸、风、伤害和黑暗的反应中积累（Braam和Davis， 1990）。TCH2蛋白与钙调蛋白有44%相同（Khan et al., 1997），表明Ca2+在TCH2功能中的作用。有人提出TCH2被Ca2+激活，并参与调节将外部刺激转化为生理反应的信号通路。为了验证这一假设，将寻找和表征TCH2突变体，以确定TCH2的生理功能。通过筛选编码以Ca2+依赖方式结合TCH2的蛋白质的基因，将探索TCH2的生化作用。这项研究将提供关于细胞如何使用Ca2+感知和响应细胞外刺激的见解。