Picking climate change winners for assisted gene flow interventions on coral reefs

挑选气候变化获胜者对珊瑚礁进行辅助基因流干预

• 批准号: 2287101
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2287101
• 关键词: Picking; climate; change; winners; assisted

One of the most important questions for biodiversity conservation in the face of global climate change is whether species will adapt, acclimatise, migrate or be extirpated. AGF involves the deliberate movement of individuals between populations within species ranges to increase the frequency of better adapted genotypes. AGF can be coupled with selective breeding of offspring from parents with certain adaptive phenotypic traits (e.g., elevated heat resistance). These approaches have been suggested as ground-breaking conservation tools in certain economically important ecosystems such as coral reefs. Both approaches require identification of "winners", i.e., naturally occurring genotypes preadapted to cope with anticipated future conditions. If successful, these techniques could buy time for coral reefs already at great risk from climate change disturbances. As part of a 5-year ERC funded project (Coralassist) we are identifying variation in heat resistance within populations of corals in Palau, Micronesia using thermal stress trials. We are examining whether selectively bred corals can be translocated to reefs in sufficiently large numbers and whether heat resistance is heritable. Using cutting edge proteomics we are identifying underlying causes of variation in heat resistance. Proteins that differ between heat stress treatments are examined using pathway analysis, (e.g., String, PathVisio) to identify biological processes, molecular functions associated with a given set of proteins and protein - protein interactions and associations. The aim of this project would be to develop tolerance indicators as it relates to protein abundance with the ultimate goal of developing markers to identify tolerant genotypes for AGF programmes without the need for heat stress trials.

面对全球气候变化，生物多样性保护最重要的问题之一是物种是否会适应、驯化、迁移或灭绝。AGF涉及物种范围内种群之间个体的故意移动，以增加更好适应基因型的频率。AGF可以与从具有某些适应性表型性状（例如，提高的耐热性）。这些方法被认为是珊瑚礁等某些具有重要经济意义的生态系统的开创性养护工具。这两种方法都需要确定“赢家”，即，自然发生的基因型预先适应科普预期的未来条件。如果成功，这些技术可以为已经面临气候变化干扰的巨大风险的珊瑚礁赢得时间。作为一个为期5年的ERC资助的项目（Coralassist）的一部分，我们正在确定在帕劳，密克罗尼西亚使用热应力试验的珊瑚种群的耐热性的变化。我们正在研究选择性繁殖的珊瑚是否可以大量转移到珊瑚礁中，以及耐热性是否可以遗传。利用最先进的蛋白质组学，我们正在确定耐热性变化的根本原因。使用途径分析来检查热应激处理之间不同的蛋白质（例如，字符串，Pathoprotein），以确定生物过程，分子功能与一组给定的蛋白质和蛋白质-蛋白质相互作用和协会。该项目的目的是开发与蛋白质丰度相关的耐受性指标，最终目标是开发标记，以确定AGF方案的耐受性基因型，而无需进行热应激试验。