REGULATION OF CONNEXIN43 GENE EXPRESSION IN THE UTERUS

描述：（改编自研究者摘要）：研究计划是 分为两部分，转录水平的调控和 在翻译水平上。该项目的第一部分是延续 研究来表征负责诱导的蛋白质， 连接蛋白43的表达其中一个的功能特性 这些蛋白质是一种新的转录因子Ini， 上一个补助期将结束。三个额外的转录 通过Southwestern blot和DNA迁移率改变鉴定了这些因素 将这些蛋白克隆到连接蛋白43基因中进行功能分析 研究的规则。可能存在额外的雌激素敏感性 连接蛋白43基因的5 '-UTR中的转录激活位点也将 追究本研究的第二部分是基于最近的 在这个实验室中发现了一个内部核糖体进入位点（IRES）， 连接蛋白43 mRNA的5 '-UTR。将研究IRES元件的功能 在体内和体外双顺反子载体构建体中。此外该 在连接蛋白43 IRES的激活中起作用的蛋白质将被 通过RNA迁移率变动分析和核糖体结合测定鉴定。

DESCRIPTION: (Adapted from the Investigator's Abstract): The research plan is divided into two parts, regulation at the transcriptional level and regulation at the translational level. The first part of the project is the continuation of studies to characterize the proteins that are responsible for the induction of connexin43 in the myometrium. The functional characterization of one of these proteins, a novel transcription factor, Ini, that was discovered during the previous grant period, will be completed. Three additional transcription factors that were identified by Southwestern blot and DNA mobility shift analysis will be cloned and the function of these proteins in connexin43 gene regulation studied. The likely presence of an additional estrogen-responsive transcriptional activation site in the 5'-UTR of the connexin43 gene will also be investigated. The second part of this research is based on the recent discovery in this laboratory of an internal ribosome entry site (IRES) in the 5'-UTR of the connexin43 mRNA. The function of the IRES element will be studied in bicistroni vector constructs both in vivo and in vitro. In addition, the proteins that function in the activation of the connexin43 IRES will be identified by RNA mobility shift analysis and ribosome binding assays.