REGULATION OF CONNEXIN43 GENE EXPRESSION IN THE UTERUS

描述：(改编自《调查者摘要》)：研究计划为 分为两部分，转录水平的调控和调控 在翻译层面上。项目的第一部分是续展 一系列研究，以确定负责诱导的蛋白质的特征 连接蛋白43在子宫肌层中的表达。其中一种的功能表征 这些蛋白质是一种新的转录因子INI，它是在 之前的授权期将结束。三个额外的转录 西南杂交和DNA迁移率漂移鉴定的因子 将克隆并分析这些蛋白在连接蛋白43基因中的功能 研究了法规。可能存在另一种雌激素反应性物质 连接蛋白43基因5‘-非编码区的转录激活位点也将 被调查。本研究的第二部分是基于最近的研究 在这个实验室中发现了一个内部核糖体进入位点(IRES) 连接蛋白43基因5‘端非编码区。将研究IRES元素的功能 在体内和体外构建Bicistroni载体。此外， 在连接蛋白43 IRES激活中起作用的蛋白质将是 经RNA迁移率漂移分析和核糖体结合分析鉴定。

DESCRIPTION: (Adapted from the Investigator's Abstract): The research plan is divided into two parts, regulation at the transcriptional level and regulation at the translational level. The first part of the project is the continuation of studies to characterize the proteins that are responsible for the induction of connexin43 in the myometrium. The functional characterization of one of these proteins, a novel transcription factor, Ini, that was discovered during the previous grant period, will be completed. Three additional transcription factors that were identified by Southwestern blot and DNA mobility shift analysis will be cloned and the function of these proteins in connexin43 gene regulation studied. The likely presence of an additional estrogen-responsive transcriptional activation site in the 5'-UTR of the connexin43 gene will also be investigated. The second part of this research is based on the recent discovery in this laboratory of an internal ribosome entry site (IRES) in the 5'-UTR of the connexin43 mRNA. The function of the IRES element will be studied in bicistroni vector constructs both in vivo and in vitro. In addition, the proteins that function in the activation of the connexin43 IRES will be identified by RNA mobility shift analysis and ribosome binding assays.