GENE EXPRESSION IN CANCER BY MICROARRAY HYBRIDIZATION

描述：(申请人描述) 这一计划的总体目标仍然是系统性和量化的 癌症基因表达的全基因组模式分析。通过使用cDNA 用于研究正常和癌症来源的人类细胞系以及 肿瘤，将扩大累积基因表达数据库，以增加 正常细胞系中基因表达模式的生物信息性 组织和肿瘤。与此同时，这样的实验将开始 刻画了许多迄今未刻画的人类的生物学角色 阵列上的基因。提出了用细胞系进行各种实验的建议。 其结果将促进对基因表达的生物学解释 在肿瘤中发现的模式。拟议的实验包括对周期的研究 在培养中生长的同步动物细胞中的基因表达； 在饥饿、高温、寒冷、 辐射等；长期基因表达变化的研究 培养和研究细胞在组织时的基因表达模式 变成基本的组织。 该计划在计算和计算方面都有实质性的改进 分析基础设施，包括进一步开发分析基础设施 算法以及改进的数据库、数据归档和数据显示 系统。最后对基因芯片技术的进一步发展进行了展望 技术的提出。

DESCRIPTION: (Applicant's Description) The overall goal of this program remains the systematic and quantitative analysis of genome-wide patterns of gene expression in cancer. By using cDNA microarrays to study normal and cancer-derived human cell lines as well as tumors, a cumulative gene expression database will be expanded to increase the biological informativeness of gene expression patterns in cell lines, normal tissues and tumors. At the same time, such experiments will begin to characterize the biological roles of the many heretofore uncharacterized human genes on the arrays. A variety of experiments with cell lines are proposed whose results will facilitate biological interpretation of gene expression patterns found in tumors. Proposed experiments include studies of periodic gene expression in synchronized animal cells growing in culture; patterns of gene expression displayed by genes when stressed with starvation, heat, cold, radiation, etc; studies of changes in gene expression during long-term culture, and studies of gene expression patterns when cells are organizing themselves into rudimentary tissues. The program includes substantial improvements in the computational and analytical infrastructure, including further development of analytical algorithms as well as improved database, data archiving and data display systems. Finally, further technology development of the cDNA microarray technology is proposed.