Matrix Otopathology

矩阵耳病理学

• 批准号: 6709487
• 负责人: Michael Anne NMI Gratton
• 金额: $ 32.73万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6709487
• 关键词: Alport syndrome; adenosine triphosphate; basement membrane; brain electrical activity; cochlea; collagen; disease /disorder model; extracellular matrix; laboratory mouse; laminin; membrane permeability; membrane potentials; noise biological effect; pathologic process; protein metabolism; proteoglycan; sodium potassium exchanging ATPase; tissue /cell culture

DESCRIPTION (provided by applicant): Basement membrane is a unique type of extracellular matrix that underlies the specialized epithelial and supporting cells of the scala media and surrounds endothelial cells and neurons. During cochlea/development, basement membranes play a critical role in cell migration and differentiation. However, the role of cochlear basement membranes post-development has not been studied. In other mature tissues, basement membranes are involved in cell adhesion and polarization as well as tissue permeability. Basement membrane formation requires assembly of a type IV collagen lattice. Other basement membrane proteins bind to the collagen lattice. A mutation in a gene encoding a type IV collagen isoform results in Alport syndrome, a disorder exhibiting progressive dysfunction of the auditory, visual and renal systems. The mouse model of Alport syndrome exhibits thickened strial capillary basement membranes. The Alport mouse is exploited to achieve the overall goal of this proposal, namely an understanding of basement membrane function in the adult cochlea. Specifically we focus on the impact of matrix thickness followed by investigation of the mechanisms controlling matrix accumulation. Experiments are designed to test three general hypotheses: 1) Strial energy metabolism is reduced in matrix otopathology. The consequences of depleted strial energy production following noise exposure are explored by characterizing electrochemical and transport properties of the stria. 2) The anionic barrier provided by basement membrane proteoglycans is increased by matrix otopathology. The proteoglycan composition of the lateral wall basement membranes is quantified with cationic probes. 3) Matrix accumulates when upregulation of synthesis exceeds degradation in collagen and laminin. Changes in the expression of genes and proteins controlling basement membrane synthesis and degradation are quantified. The proposed work clarifies the relationship of basement membrane to strial function and normal hearing.

描述（由申请人提供）：基底膜是一种独特类型的细胞外基质，位于scala介质的特化上皮细胞和支持细胞之下，并包围内皮细胞和神经元。在耳蜗发育过程中，基底膜在细胞迁移和分化中起着至关重要的作用。然而，耳蜗基底膜在发育后的作用尚未得到研究。在其他成熟组织中，基底膜参与细胞粘附和极化以及组织渗透性。基底膜的形成需要IV型胶原晶格的组装。其他基底膜蛋白与胶原晶格结合。编码IV型胶原异构体的基因突变导致阿尔波特综合征，这是一种表现为听觉、视觉和肾脏系统进行性功能障碍的疾病。Alport综合征小鼠模型毛细血管基底膜增厚。利用Alport小鼠来实现本提案的总体目标，即了解成人耳蜗的基底膜功能。我们特别关注基质厚度的影响，然后研究控制基质积累的机制。实验旨在验证三个一般假设：1)基质耳病理学中试验能量代谢减少。通过表征条纹的电化学和输运特性，探讨了噪声暴露后耗尽试验能量产生的后果。基底膜蛋白聚糖提供的阴离子屏障通过基质耳病理增加。用阳离子探针定量测定侧壁基底膜的蛋白多糖组成。3)当胶原蛋白和层粘连蛋白的合成上调超过降解时，基质积累。控制基底膜合成和降解的基因和蛋白质表达的变化被量化。