Molecular Diversity and Function of the Receptor Dscam

受体 Dscam 的分子多样性和功能

• 批准号: 6755055
• 负责人: DIETMAR SCHMUCKER
• 金额: $ 40.34万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6755055
• 关键词: Downs syndrome; Drosophilidae; cell adhesion molecules; genetic library; green fluorescent proteins; ligands; neuronal guidance; polymerase chain reaction; protein isoforms; protein structure function; receptor expression; synaptogenesis

DESCRIPTION (provided by applicant): We have recently identified the Drosophila receptor Dscam and shown that it is essential for the formation of proper connectivity in the fly nervous system. Dscam is a member of the immunoglobulin superfamily and is highly related to the human protein "Down syndrome cell adhesion molecule" (DSCAM). The Drosophila Dscam gene is extraordinarily complex and can generate some 38,000-protein isoforms through alternative splicing. The overall goal of our studies is to test the hypothesis that alternative splice isoforms of the Dscam receptor constitute a molecular recognition code for synaptic specificity. We will use genetic and molecular approaches to functionally test the importance of neuronal receptor diversity. We propose that the extraordinary molecular diversity of Dscam provides a paradigmatic and genetically accessible case for systematically dissecting the mechanism by which neuronal receptors contribute to synaptic specificity.

描述（由申请人提供）：我们最近确定了果蝇受体DSCAM，并表明这对于在蝇神经系统中形成适当的连通性至关重要。 DSCAM是免疫球蛋白超家族的成员，与人类蛋白“唐氏综合征细胞粘附分子”（DSCAM）高度相关。果蝇DSCAM基因非常复杂，可以通过替代剪接产生约38,000个蛋白质同工型。我们研究的总体目标是检验以下假设：DSCAM受体的替代剪接同工型构成了突触特异性的分子识别代码。我们将使用遗传和分子方法在功能上测试神经元受体多样性的重要性。我们建议DSCAM的特殊分子多样性为系统解剖神经元受体有助于突触特异性的机制提供了一种范式且遗传上可访问的病例。