Proteome Markers for Alzheimer's Disease Diagnosis
Proteome Markers for Alzheimer's Disease Diagnosis
批准号:
6721495
负责人:
KELVIN H LEE
金额:
$23.92万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-04-01 至 2007-03-31
中文摘要
描述(改编自申请人摘要):该项目的目的
英文摘要
DESCRIPTION (Adapted from applicant's abstract): The objective of this project
is to identify CSF molecular markers associated with the antemortem diagnosis
of Alzheimer's disease which can be used as a measure for disease pathology. In
contrast to brain based studies of the molecular mechanism of disease
progression, this investigation with use proteomics technology to identify and
characterize CSF proteins of interest. This information can be used to
categorize disease pathology or can be used to develop immunoassays for
diagnosis. In order to achieve these long-term objectives, the investigator
plans to: identify and obtain CSF blood samples and clinical information from
an appropriate sample population of patients with different forms of dementia,
probable Alzheimer's disease at various stages and controls (Specific Aim 1).
Analyze the samples for AD specific protein markers used in proteome analysis
that includes high solution two-dimensional electrophoresis, laser
densitometry, fluorescence imaging, and computer aided in each analysis
(Specific Aim 2). He employs multivariate statistics to establish a correlation
between relevant changes in disease diagnosis and establish qualitative and
quantitative bar code for diagnosis of Alzheimer's disease. This includes the
validation of other proposed CSF markers such as tau and Abeta 42 as well as
the preliminary identification of other major differential diagnosis such as
vascular dementia, dementia with Lewy bodies (Specific Aim 3). Use
microchemical characterization of important proteins to elucidate the genetic
basis of the molecular markers and other reference proteins. This information
can also be used to develop immunoassays (Specific Aim 4).
期刊论文(11)
专著(0)
科研奖励(0)
会议论文
Alzheimer's disease cerebrospinal fluid biomarker discovery: a proteomics approach.
阿尔茨海默病脑脊液生物标志物的发现:蛋白质组学方法。
DOI:
--
发表时间:
2005
期刊:
Current opinion in molecular therapeutics.
影响因子:
--
作者:
[D'Ascenzo,Mark, Relkin,NormanR, Lee,KelvinH]
通讯作者:
Lee,KelvinH
Two-dimensional protein electrophoresis: from molecular pathway discovery to biomarker discovery in neurological disorders.
二维蛋白质电泳:从分子途径发现到神经系统疾病生物标志物发现。
DOI:
10.1016/j.nurx.2006.05.001
发表时间:
2006
期刊:
NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics.
影响因子:
--
作者:
[Choe,LeilaH, Werner,BrendaG, Lee,KelvinH]
通讯作者:
Lee,KelvinH
DOI:
10.1155/2005/806573
发表时间:
2005
期刊:
Disease markers
影响因子:
--
作者:
[Finehout EJ, Franck Z, Lee KH]
通讯作者:
Lee KH
PROTEASE DIGEST CONDITIONS FOR MEMBRANE PROTEIN PROTEOMICS
-
批准号:8364949
-
项目类别:
-
资助金额:$6.39万
-
财政年份:2011
-
负责人:KELVIN H LEE
-
依托单位:
Proteome Markers for Alzheimer's Disease Diagnosis
-
批准号:6324151
-
项目类别:
-
资助金额:$35.05万
-
财政年份:2001
-
负责人:KELVIN H LEE
-
依托单位:
Proteome Markers for Alzheimer's Disease Diagnosis
-
批准号:6538949
-
项目类别:
-
资助金额:$23.63万
-
财政年份:2001
-
负责人:KELVIN H LEE
-
依托单位:
Proteome Markers for Alzheimer's Disease Diagnosis
-
批准号:6639119
-
项目类别:
-
资助金额:$23.82万
-
财政年份:2001
-
负责人:KELVIN H LEE
-
依托单位:
国内基金
海外基金
新型F-18标记香豆素衍生物PET探针的研制及靶向Alzheimer's Disease 斑块显像研究
-
批准号:81000622
-
项目类别:青年科学基金项目
-
资助金额:20.0万元
-
批准年份:2010
-
负责人:梁胜
-
依托单位:
阿尔茨海默病(Alzheimer's disease,AD)动物模型构建的分子机理研究
-
批准号:31060293
-
项目类别:地区科学基金项目
-
资助金额:26.0万元
-
批准年份:2010
-
负责人:郭亚芬
-
依托单位:
跨膜转运蛋白21(TMP21)对引起阿尔茨海默病(Alzheimer'S Disease)的γ分泌酶的作用研究
-
批准号:30960334
-
项目类别:地区科学基金项目
-
资助金额:22.0万元
-
批准年份:2009
-
负责人:董贵成
-
依托单位: