Identification of the circadian clock proteome

生物钟蛋白质组的鉴定

• 批准号: 6835236
• 负责人: JASON P DEBRUYNE
• 金额: $ 4.73万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6835236
• 关键词: 3T3 cells; RNA interference; biological clocks; biotechnology; circadian rhythms; gene expression; genetic regulation; immunocytochemistry; laboratory mouse; postdoctoral investigator; protein protein interaction; protein quantitation /detection; protein structure function; proteomics; seasonal affective disorder; sleep disorders; transfection

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to identify the components and mechanisms involved in regulating circadian patterns of clock gene expression in mammals. Mammalian circadian clock proteins are found in huge complexes, that differ in composition at various times of day. A proteomics based approach will be used to identify unknown proteins bound in these complexes, in an in vitro cellular context. Identified proteins will be evaluated for in vivo interactions to confirm the identified interactions are biologically relevant. The functions and necessity of the biologically interacting proteins within the mammalian circadian clock will then be determined. Thus, this work will identify novel circadian clock proteins, which may serve as therapeutic targets for ameliorating clock related ailments (e.g. jet lag, shift work) and more serious conditions such as circadian rhythm sleep disorders and other clock-related psychological disorders (e.g. seasonal affective disorder).

描述（由申请人提供）：本提案的长期目标是确定参与调节哺乳动物生物钟基因表达的昼夜节律模式的组分和机制。哺乳动物的生物钟蛋白质被发现在巨大的复合体中，在一天的不同时间组成不同。基于蛋白质组学的方法将用于在体外细胞环境中鉴定结合在这些复合物中的未知蛋白质。将评价已鉴别蛋白质的体内相互作用，以确认已鉴别相互作用具有生物学相关性。然后将确定哺乳动物生物钟内生物相互作用蛋白质的功能和必要性。因此，这项工作将确定新的生物钟蛋白，这可能作为改善生物钟相关疾病（如时差，轮班工作）和更严重的条件，如昼夜节律睡眠障碍和其他生物钟相关的心理障碍（如季节性情感障碍）的治疗目标。