Identification of the circadian clock proteome

描述（由申请人提供）：该提案的长期目标是确定参与调节哺乳动物时钟基因表达昼夜节律模式的成分和机制。哺乳动物生物钟蛋白存在于巨大的复合体中，其在一天中不同时间的组成不同。基于蛋白质组学的方法将用于在体外细胞环境中识别这些复合物中结合的未知蛋白质。将评估已鉴定的蛋白质的体内相互作用，以确认已鉴定的相互作用具有生物学相关性。然后将确定哺乳动物生物钟内生物相互作用蛋白质的功能和必要性。因此，这项工作将鉴定新的生物钟蛋白，它们可能作为改善生物钟相关疾病（例如时差、轮班工作）和更严重的疾病（例如昼夜节律睡眠障碍和其他生物钟相关心理障碍（例如季节性情感障碍））的治疗靶点。

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to identify the components and mechanisms involved in regulating circadian patterns of clock gene expression in mammals. Mammalian circadian clock proteins are found in huge complexes, that differ in composition at various times of day. A proteomics based approach will be used to identify unknown proteins bound in these complexes, in an in vitro cellular context. Identified proteins will be evaluated for in vivo interactions to confirm the identified interactions are biologically relevant. The functions and necessity of the biologically interacting proteins within the mammalian circadian clock will then be determined. Thus, this work will identify novel circadian clock proteins, which may serve as therapeutic targets for ameliorating clock related ailments (e.g. jet lag, shift work) and more serious conditions such as circadian rhythm sleep disorders and other clock-related psychological disorders (e.g. seasonal affective disorder).