Models for Iron Active Sites of Nitrogenases

固氮酶铁活性位点模型

• 批准号: 6836615
• 负责人: MARK P. MEHN
• 金额: $ 4.11万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6836615
• 关键词: X ray crystallography; active sites; amines; biomimetics; catalyst; chemical kinetics; chemical models; chemical reaction; conformation; intermolecular interaction; iron; iron compounds; model design /development; nitrene; nitrogen metabolism; nitrogenase; nuclear magnetic resonance spectroscopy; postdoctoral investigator; protein structure function; spectrometry; structural biology; thermodynamics

DESCRIPTION (provided by applicant): Bioinspired functional iron model complexes related to nitrogenase will be generated focusing on the activation of dinitrogen. Key reduced nitrogen intermediates in a Chatt-type cycle will be isolated and the physical and spectroscopic properties of these compounds examined. The basic physical properties of iron nitrides, nitrenes, diazenidos, hydrazido, amido and amine functionalities are desired. Initially a series of strong field ligands will be examined. Low valent iron complexes of these ligands are capable of activating dinitrogen, but the subsequent reactivity has not been explored. Of particular interest are iron (IV)-nitrides for their applicability to group transfer processes. A number of spectroscopic studies will be undertaken to detail the electronic structures of these unique intermediates. The mechanism of formation and decay of metal-dinitrogen intermediates will be studied via kinetic and spectroscopic methods. In the next phase, sulfur rich ligands will be examined and the possibility of the application to group transfer processes will be examined. Through the principles of nitrogen activation gained from these biomimetic complexes, greater understanding of the role of iron in nitrogenase will be gained.

描述（由申请人提供）：将产生与氮酶相关的生物启发功能铁模型复合物，重点是二氮的活化。将分离chatt型循环中的关键还原氮中间体，并检查这些化合物的物理和光谱特性。氮化铁、亚硝基、重氮基、肼基、胺基和胺基的基本物理性质是必需的。首先，我们将研究一系列强场配体。这些配体的低价铁配合物能够激活二氮，但随后的反应性尚未探索。特别令人感兴趣的是铁(IV)-氮化物，因为它们适用于基团转移过程。将进行一些光谱学研究，以详细说明这些独特中间体的电子结构。本文将通过动力学和光谱学方法研究金属-二氮中间体的形成和衰变机理。在下一阶段，将研究富硫配体，并研究其应用于基团转移过程的可能性。通过从这些仿生复合物中获得的氮活化原理，将对铁在氮酶中的作用有更深入的了解。