Nematode gonad development and the evolution of organs

线虫性腺发育和器官的进化

• 批准号: 6859013
• 负责人: DAVID J RUDEL
• 金额: $ 0.55万
• 依托单位: MAX PLANCK INST FOR DEVELOPMENTL BIOLOGY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6859013
• 关键词: Caenorhabditis elegans; Nematoda; developmental genetics; germ cells; gonads; hermaphroditism; molecular cloning; morphology; postdoctoral investigator; reproductive development; scanning electron microscopy; transmission electron microscopy

DESCRIPTION (provided by applicant): The long-term objective of this research is to understand how organisms evolve at a molecular level. The short-term goal is to understand gonad development in the nematode Pristionchus pacificus. The nematode gonad is a complex organ composed of both somatic and germline tissues. To form a functional organ, the right number of cells must be made, the right cell-types specified, and ceils must rearrange to form functional tissues of the right size in the correct location. Many of these processes are controlled by similar pathways and molecules in all organisms, whether worm or human. When these pathways are aberrantly controlled, the results are often detrimental. However, changes in developmental processes must occur to allow for the generation of species. It is not known how plastic fundamental processes are. Much effort has established P. pacificus as a second genetic model nematode for comparison with C. elegans. Investigation of gonad development in P. pacificus offers the opportunity to study change in many developmental processes through comparisons. The gonad of P. pacificus has gross morphological differences in comparison to C. elegans. Over the next three years, the P. pacificus gonad will be characterized and genes involved in gonad development will be isolated. Classical lineaging techniques and laser ablations will be used to fate map the gonad. Cell contacts will be further described using electron micrographs. Concurrently, unbiased genetic screens will uncover involved loci. Mutants will be classified by phenotype and according to the characterization of the gonad and inferred cell interactions. Comparison with C. elegans will begin to dissect how the processes that form the nematode gonad and germ line have changed. Ultimately, insights into the pattern of molecular changes that can occur and may have resulted in the varied metazoans we know may be gained.

描述（由申请人提供）： 这项研究的长期目标是了解生物体如何在分子水平上进化。短期目标是了解太平洋棱纹线虫的性腺发育。线虫性腺是一个由体细胞和生殖细胞组成的复杂器官。为了形成一个功能器官，必须制造正确数量的细胞，指定正确的细胞类型，并且细胞必须重新排列以在正确的位置形成正确大小的功能组织。在所有生物体中，无论是蠕虫还是人类，这些过程中的许多都是由类似的途径和分子控制的。当这些途径受到异常控制时，结果往往是有害的。然而，发育过程必须发生变化，才能产生物种。塑料的基本过程是怎样的，我们还不知道。许多努力已经将太平洋原甲线虫作为第二种遗传模式线虫，用于与C.优雅的。对太平洋对虾性腺发育的研究为通过比较研究许多发育过程中的变化提供了机会。太平洋对虾的性腺在形态上与中国对虾有较大的差异。优雅的。在接下来的三年里，太平洋对虾的性腺将被表征，参与性腺发育的基因将被分离。经典的谱系技术和激光消融将被用来命运图的性腺。将使用电子显微照片进一步描述电池接触。同时，无偏的遗传筛选将发现相关的基因座。突变体将通过表型并根据性腺和推断的细胞相互作用的表征进行分类。与C.线虫将开始剖析形成线虫性腺和生殖系的过程是如何改变的。最终，我们可能会了解到可能发生的分子变化的模式，并可能导致我们所知道的各种后生动物。