The Role of Chromatin Modification During Development

染色质修饰在发育过程中的作用

• 批准号: 6738245
• 负责人: Johnathan R. Whetstine
• 金额: $ 4.3万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6738245
• 关键词: Caenorhabditis elegans; RNA interference; amidohydrolases; chromatin; chromatin immunoprecipitation; developmental genetics; embryogenesis; enzyme activity; enzyme mechanism; gene induction /repression; genetic regulation; microarray technology; polymerase chain reaction; postdoctoral investigator

DESCRIPTION (provided by applicant): Chromatin modification has recently emerged as a novel and important mechanism in the regulation of gene expression. However, the impact that chromatin modifiers and the resulting modifications have on molecular networks establishing proper cell-fate and development in multi-cellular organisms are just beginning to emerge. In order to better understand this question, this NRSA will use the C. elegans as an in vivo system to investigate the role of HDA-1 (histone-deacetylase 1) during early embryogenesis by using molecular, biochemical, and genomic approaches. Since HDA-1 is crucial for proper cell-fate determination, the identification of direct HDA-1 target genes and the impact that this protein has on the chromatin environment will be fundamental in understanding the mechanistic role of HDA-1. Based on these exciting findings, new models are likely to emerge about the in vivo function and mechanism of action of histone deacetylases in the development of a multi-cellular organism.

描述（由申请人提供）：