The Role of Chromatin Modification During Development

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DESCRIPTION (provided by applicant): Chromatin modification has recently emerged as a novel and important mechanism in the regulation of gene expression. However, the impact that chromatin modifiers and the resulting modifications have on molecular networks establishing proper cell-fate and development in multi-cellular organisms are just beginning to emerge. In order to better understand this question, this NRSA will use the C. elegans as an in vivo system to investigate the role of HDA-1 (histone-deacetylase 1) during early embryogenesis by using molecular, biochemical, and genomic approaches. Since HDA-1 is crucial for proper cell-fate determination, the identification of direct HDA-1 target genes and the impact that this protein has on the chromatin environment will be fundamental in understanding the mechanistic role of HDA-1. Based on these exciting findings, new models are likely to emerge about the in vivo function and mechanism of action of histone deacetylases in the development of a multi-cellular organism.