Core--Biochemistry

生物化学核心实验室为本计划拨款申请中描述的单个项目提供分析支持。这里描述的调查所需的大多数分析物是通过放射免疫测定法（RIA），酶免疫测定法（EIA）和酶测定法测量的。通过在配备合适仪器的实验室中，由具有长期RIA、EIA和酶技术经验的工作人员分析个体研究者生成的所有样品，可以以更统一的方式获得数据，并具有最严格的质量控制和最低的试剂费用。来自几个调查人员的样品可以在一次分析运行中分析，从而防止了由单独的调查人员分析少量样品所造成的浪费。此外，可以更有效地安排工作，以便技术人员的时间得到最大效率的利用，几乎没有空闲时间。几个研究者的项目同时进行，从而降低了试剂成本，减少了人工浪费，提供了更统一的结果。生物化学核心实验室的作用是通过处理和分析各种项目产生的样品，为研究人员提供分析支持，保持质量控制和熟练程度，并减少试剂和人员费用。通过集中进行样品处理和分析，实验室可以帮助每个研究者掌握适当的收集技术、样本量和其他可能影响给定分析成功的分析前因素。质量控制，技术

The Biochemistry Core Laboratory provides analytical support for the individual projects described in this Program grant application. The majority of the analytes required by the investigations described here are measured by radioimmunoassay (RIA), enzyme immunoassay (EIA), and enzymatic assays. By having all the samples generated by individual investigators analyzed by staff with long experience with RIA, EIA, and enzymatic techniques, in laboratories equipped with suitable instruments, data can be obtained in a more uniform manner, with the strictest possible quality control and minimum of reagent expense. Samples from several investigators can be analyzed within one assay run, thus preventing the wastage which would be incurred by separate investigators analyzing small numbers of samples. Additionally, work can be scheduled more efficiently so that the technologists' time is utilized with maximum efficiency resulting in almost no slack time. The projects of several investigators are worked on at the same time, thus reducing reagent costs, waste of labor and providing more uniform results. The role of the Biochemistry Core Laboratory is to provide analytical support to the investigators in this application by processing and analyzing the samples generated by the various projects, maintain quality control and proficiency, and reduce reagent and personnel expense. By centralizing the performance of sample processing and analysis, the laboratory can assist each investigator with the proper collection techniques, sample size and other pre-analytic factors that may influence the success of a given analysis. Quality control, technical proficiency, and equipment performance can be easily monitored to assure the accuracy of results. Therefore, personnel and reagents can be utilized more efficiently while maintaining the quality of the analyses. The centralized core helps evaluate and implement new technologies and equipment which influence the results of this project. The Core's development of the Nova 1(TM) Electrolyte Analyzer and the Luminex (TM) Protein Multiple Immunoassay helps increase efficiency and productivity.