Core--Biochemistry

生物化学核心实验室为本计划拨款申请中描述的各个项目提供分析支持。这里描述的研究所需的大多数分析物是通过放射免疫测定 (RIA)、酶免疫测定 (EIA) 和酶测定来测量的。通过由具有 RIA、EIA 和酶技术长期经验的工作人员在配备合适仪器的实验室中分析个别研究人员产生的所有样本，可以以更统一的方式获得数据，并进行最严格的质量控制和最低的试剂费用。来自多个研究人员的样品可以在一次测定运行中进行分析，从而防止因单独的研究人员分析少量样品而造成的浪费。此外，可以更有效地安排工作，从而最大限度地利用技术人员的时间，从而几乎没有空闲时间。多个研究人员的项目同时进行，从而减少试剂成本、劳动力浪费并提供更统一的结果。生物化学核心实验室的作用是通过处理和分析各个项目生成的样本，为该应用中的研究人员提供分析支持，保持质量控制和熟练程度，并减少试剂和人员费用。通过集中样品处理和分析的性能，实验室可以帮助每位研究者采用适当的采集技术、样品大小和其他可能影响给定分析成功的分析前因素。质量控制、技术 可以轻松监控熟练程度和设备性能，以确保结果的准确性。因此，可以更有效地利用人员和试剂，同时保持分析质量。集中核心有助于评估和实施影响该项目结果的新技术和设备。 Core 开发的 Nova 1(TM) 电解质分析仪和 Luminex(TM) 蛋白质多重免疫测定有助于提高效率和生产力。

The Biochemistry Core Laboratory provides analytical support for the individual projects described in this Program grant application. The majority of the analytes required by the investigations described here are measured by radioimmunoassay (RIA), enzyme immunoassay (EIA), and enzymatic assays. By having all the samples generated by individual investigators analyzed by staff with long experience with RIA, EIA, and enzymatic techniques, in laboratories equipped with suitable instruments, data can be obtained in a more uniform manner, with the strictest possible quality control and minimum of reagent expense. Samples from several investigators can be analyzed within one assay run, thus preventing the wastage which would be incurred by separate investigators analyzing small numbers of samples. Additionally, work can be scheduled more efficiently so that the technologists' time is utilized with maximum efficiency resulting in almost no slack time. The projects of several investigators are worked on at the same time, thus reducing reagent costs, waste of labor and providing more uniform results. The role of the Biochemistry Core Laboratory is to provide analytical support to the investigators in this application by processing and analyzing the samples generated by the various projects, maintain quality control and proficiency, and reduce reagent and personnel expense. By centralizing the performance of sample processing and analysis, the laboratory can assist each investigator with the proper collection techniques, sample size and other pre-analytic factors that may influence the success of a given analysis. Quality control, technical proficiency, and equipment performance can be easily monitored to assure the accuracy of results. Therefore, personnel and reagents can be utilized more efficiently while maintaining the quality of the analyses. The centralized core helps evaluate and implement new technologies and equipment which influence the results of this project. The Core's development of the Nova 1(TM) Electrolyte Analyzer and the Luminex (TM) Protein Multiple Immunoassay helps increase efficiency and productivity.