Core--Biochemistry

核心--生物化学

• 批准号: 7386020
• 负责人: Kathleen Bridget Brosnihan
• 金额: $ 22.18万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7386020
• 关键词: Applications Grants; Biochemistry; Biological Assay; Collection; Data; Development; Electrolytes; Enzyme Immunoassay; Equipment; Human Resources; Immunoassay; Individual; Investigation; Laboratories; Measures; Monitor; Numbers; Performance; Process; Productivity; Proteins; Quality Control; Radioimmunoassay; Reagent; Research Personnel; Role; Running; Sample Size; Sampling; Schedule; Techniques; Time; Work; cost; experience; instrument; new technology; prevent; programs; success; wasting

The Biochemistry Core Laboratory provides analytical support for the individual projects described in this Program grant application. The majority of the analytes required by the investigations described here are measured by radioimmunoassay (RIA), enzyme immunoassay (EIA), and enzymatic assays. By having all the samples generated by individual investigators analyzed by staff with long experience with RIA, EIA, and enzymatic techniques, in laboratories equipped with suitable instruments, data can be obtained in a more uniform manner, with the strictest possible quality control and minimum of reagent expense. Samples from several investigators can be analyzed within one assay run, thus preventing the wastage which would be incurred by separate investigators analyzing small numbers of samples. Additionally, work can be scheduled more efficiently so that the technologists' time is utilized with maximum efficiency resulting in almost no slack time. The projects of several investigators are worked on at the same time, thus reducing reagent costs, waste of labor and providing more uniform results. The role of the Biochemistry Core Laboratory is to provide analytical support to the investigators in this application by processing and analyzing the samples generated by the various projects, maintain quality control and proficiency, and reduce reagent and personnel expense. By centralizing the performance of sample processing and analysis, the laboratory can assist each investigator with the proper collection techniques, sample size and other pre-analytic factors that may influence the success of a given analysis. Quality control, technical proficiency, and equipment performance can be easily monitored to assure the accuracy of results. Therefore, personnel and reagents can be utilized more efficiently while maintaining the quality of the analyses. The centralized core helps evaluate and implement new technologies and equipment which influence the results of this project. The Core's development of the Nova 1(TM) Electrolyte Analyzer and the Luminex (TM) Protein Multiple Immunoassay helps increase efficiency and productivity.

生物化学核心实验室为本计划拨款申请中描述的个别项目提供分析支持。这里描述的调查所需的大多数分析物是通过放射免疫分析(RIA)、酶免疫分析(EIA)和酶分析来测量的。通过在配备适当仪器的实验室中，由具有长期RIA、EIA和酶技术经验的工作人员分析由个别调查人员产生的所有样本，可以以更统一的方式获得数据，并尽可能严格地进行质量控制，并将试剂费用降至最低。来自多个调查人员的样本可以在一次化验运行中进行分析，从而避免了单独的调查人员分析少量样本所造成的浪费。此外，可以更高效地安排工作，从而最大限度地利用技术人员的时间，从而几乎没有空闲时间。多个研究人员的项目同时进行，从而降低了试剂成本，浪费了劳动力，并提供了更一致的结果。生物化学核心实验室的作用是通过处理和分析各种项目产生的样本，为这一应用中的研究人员提供分析支持，保持质量控制和熟练程度，并减少试剂和人员费用。通过集中样本处理和分析的性能，实验室可以协助每个研究人员掌握适当的收集技术、样本大小和其他可能影响特定分析成功的分析前因素。质量控制，技术 熟练程度和设备性能可以很容易地监控，以确保结果的准确性。因此，在保持分析质量的同时，可以更有效地利用人员和试剂。集中的核心有助于评估和实施影响该项目结果的新技术和设备。酷睿公司开发的Nova 1(TM)电解质分析仪和Luminex(TM)蛋白质多重免疫分析有助于提高效率和生产率。