Spectratyping Core

光谱分型是一种基于TCR受体利用率来测量T细胞反应的快速方法。它包括根据应答T细胞表达的V基因以及抗原接触区的长度来划分应答T细胞，该抗原接触区被称为第三互补决定区CDR3。该技术包括扩增其中一条TCR链的对应于mRNA的cDNA，并在变性凝胶上分离扩增产物。通常，正常曲目由对应于不同CDR3长度的多个带表示，其强度服从泊松分布。在抗原的刺激下，对抗原有反应的细胞的TCR条带显示出更强的强度。 我们预计核心实验室每年将进行1000多次光谱测试，以支持这三个项目，并支持技术目标1，也许还有一些试点项目。通过集中这些分析，我们将允许单个项目负责人将重点放在他们正在进行的研究的其他方面。此外，核心将保证中心所有研究部分的数据集是可复制的。

Spectratyping is a rapid method for measuring T cell responses on the basis of TCR receptor utilization. It consists of dividing the responding T cell on the basis of the V gene they express as well as the length of the antigen contact region referred to as the third complementarity determining region, CDR3. The technique consists of amplifying the cDNA corresponding to mRNA for one of the TCR chains and separating the amplification product on denaturing gels. Typically, a normal repertoire is represented by multiple bands corresponding to different CDR3 lengths, whose intensity follows a Poisson distribution. Upon stimulation with antigen, bands corresponding to TCR from cells that responded to the antigen show increased intensity. We expect that the core laboratory will perform over 1000 spectratypes/year in support of these three projects as well as in support of technical Aim 1 and perhaps some of the pilot projects. By centralizing these analyses we will allow the individual project leaders to focus on other aspects of the research they are pursuing. In addition the Core will guarantee reproducible data sets for all the research components of the Center.