Genomics of Gene Regulation in Porphyromonas gingivalis
牙龈卟啉单胞菌基因调控的基因组学
基本信息
- 批准号:6795202
- 负责人:
- 金额:$ 53.68万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-04-01 至 2007-12-31
- 项目状态:已结题
- 来源:
- 关键词:Bacteroides gingivalisbacteria infection mechanismbacterial geneticsbioinformaticsbiological signal transductionchromatin immunoprecipitationclinical researchdental plaquefunctional /structural genomicsgel mobility shift assaygene environment interactiongene expressiongene expression profilinggenetic promoter elementgenetic regulationgenetic transcriptiongingivahost organism interactionmicroarray technologymixed tissue /cell culturemolecular geneticsnucleic acid sequenceoral bacteriapatient oriented researchperiodontitis
项目摘要
DESCRIPTION (provided by applicant): Deciphering the "conversation" between bacterium and host during infection is central to understanding microbial pathogenesis and host defense. Periodontal diseases have long been recognized as bacterial infections, and a long-term goal of the laboratory is to identify P. gingivalis genes expressed during infection, ie. one side of the cross-talk between bacterium and host. The genetic tractability of the organism, combined with the availability of a completed genome sequence and gene microarrays, has made this goal approachable. Bacteria use two-component signal transduction systems to monitor and react to changes in their environment. In this application, we test the hypothesis that in P. gingivalis these systems are responsive to in vivo conditions prevailing in the subgingival crevice during health and disease. A comprehensive study is proposed of the molecular mechanisms of gene regulation in P. gingivalis, a new area of research with this organism. Response regulators will be analyzed to identify the genes they control, and their promoter targets. We will test our hypothesis by determining the expression of a subset of identified genes in the in vivo environment of subgingival plaque from periodontitis patients. The experimental approaches exploit our experience in molecular genetics, whole genome transcription profiling, promoter target localization, and DNA sequence analysis. The goals are responsive to the RFA since we will analyze P. gingivalis intracellular signaling mechanisms that orchestrate responses to environmental changes. By defining the regulators, the genes they control, and their in vitro and in vivo expression patterns, we can identify conditions that may trigger periodontitis, and target rational therapeutic interventions to the regulatory molecular switches.
描述(由申请人提供):破译感染过程中细菌和宿主之间的“对话”是理解微生物发病机制和宿主防御的核心。牙周病长期以来一直被认为是细菌感染,实验室的一个长期目标是鉴定感染过程中表达的牙龈假单胞菌基因,即。细菌和宿主之间相互作用的一面。生物体的遗传易操纵性,再加上可获得完整的基因组序列和基因微阵列,使这一目标变得容易实现。细菌使用两组分的信号转导系统来监测环境的变化并对其做出反应。在这项应用中,我们测试假设,在牙龈假单胞菌中,这些系统对健康和疾病期间在牙龈下缝隙中普遍存在的活体条件有反应。对牙龈假单胞菌这一新的研究领域的基因调控分子机制进行了全面的研究。将对反应调节因子进行分析,以确定它们控制的基因和它们的启动子目标。我们将通过确定牙周炎患者牙周下菌斑的活体环境中已识别基因的子集的表达来验证我们的假设。这些实验方法利用了我们在分子遗传学、全基因组转录图谱、启动子靶标定位和DNA序列分析方面的经验。这些目标是对RFA的反应,因为我们将分析牙龈假单胞菌细胞内协调对环境变化反应的信号机制。通过定义调控因子及其控制的基因,以及它们在体外和体内的表达模式,我们可以识别可能引发牙周炎的条件,并针对调控分子开关进行合理的治疗干预。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(3)
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Margaret J Duncan其他文献
Margaret J Duncan的其他文献
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{{ truncateString('Margaret J Duncan', 18)}}的其他基金
Physiological Interaction Between Probiotic Bacteria and Porphyromonas gingivalis
益生菌与牙龈卟啉单胞菌之间的生理相互作用
- 批准号:
8685401 - 财政年份:2014
- 资助金额:
$ 53.68万 - 项目类别:
Physiological Interaction Between Probiotic Bacteria and Porphyromonas gingivalis
益生菌与牙龈卟啉单胞菌之间的生理相互作用
- 批准号:
9221995 - 财政年份:2014
- 资助金额:
$ 53.68万 - 项目类别:
Physiological Interaction Between Probiotic Bacteria and Porphyromonas gingivalis
益生菌与牙龈卟啉单胞菌之间的生理相互作用
- 批准号:
9002031 - 财政年份:2014
- 资助金额:
$ 53.68万 - 项目类别:
Genomics of Gene Regulation in Porphyromonas gingivalis
牙龈卟啉单胞菌基因调控的基因组学
- 批准号:
6871337 - 财政年份:2004
- 资助金额:
$ 53.68万 - 项目类别:
Genomics of Gene Regulation in P. gingivalis
牙龈卟啉单胞菌基因调控的基因组学
- 批准号:
8112651 - 财政年份:2004
- 资助金额:
$ 53.68万 - 项目类别:
Genomics of Gene Regulation in P. gingivalis
牙龈卟啉单胞菌基因调控的基因组学
- 批准号:
7664566 - 财政年份:2004
- 资助金额:
$ 53.68万 - 项目类别:
Genomics of Gene Regulation in Porphyromonas gingivalis
牙龈卟啉单胞菌基因调控的基因组学
- 批准号:
7161363 - 财政年份:2004
- 资助金额:
$ 53.68万 - 项目类别:
Genomics of Gene Regulation in P. gingivalis
牙龈卟啉单胞菌基因调控的基因组学
- 批准号:
7897918 - 财政年份:2004
- 资助金额:
$ 53.68万 - 项目类别:
Genomics of Gene Regulation in P. gingivalis
牙龈卟啉单胞菌基因调控的基因组学
- 批准号:
8299177 - 财政年份:2004
- 资助金额:
$ 53.68万 - 项目类别:
Genomics of Gene Regulation in P. gingivalis
牙龈卟啉单胞菌基因调控的基因组学
- 批准号:
7524478 - 财政年份:2004
- 资助金额:
$ 53.68万 - 项目类别: