Detection And Identification Of Mycobacteria In Clinical

临床分枝杆菌的检测与鉴定

基本信息

  • 批准号:
    6675201
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
  • 资助国家:
    美国
  • 起止时间:
  • 项目状态:
    未结题

项目摘要

Detection and identification of acid-fast bacilli of Mycobacterium species by conventional procedures requires growing the organisms from patient specimens and then testing the isolates for various phenotypic characteristics. These methods may take days to 1 or more months. The development of a few highly specific molecular probes for testing cultures growing acid-fast bacilli has greatly reduced the time to identification of some mycobacterial isolates. Recently, the polymerase chain reaction and isothermal nucleic acid amplification techniques have been used in assays that offer a high degree of specificity and reasonable sensitivity for detection of Mycobacterium tuberculosis in clinical samples. It would be useful to have amplification assay systems that are capable of detecting multiple Mycobacterium species while excluding cross-reactive signals from other bacteria commonly present in clinical samples. Experiments have been successfully performed with an assay version that simultaneously detects the presence or absence of nucleic acid amplification products from six common clinically isolated Mycobacterium species. A joint patent application between the N.I.H. and Beckman Coulter Corporation has been recently submitted. Because sample preparation is a critical component of molecular diagnostic assays, we have initiated a new effort to investigate the usefulness of a pressure cycling technology (PCT) for improving the efficiency of nucleic acid release from mycobacterium cells. A barocycler (device for pressure cycling) has been recently brought into CPD under a CRADA with BBI Biotech. Using PCT as a pretreatment before nucleic acid extraction we were able to increase the average PCR signals from suspensions containing Mycobacterium gordonae and suspensions of Mycobacterium tuberculosis. A collaborative prospective study is being initiated with the Johns Hopkins Center for TB Research and BBI to evaluate the utility of different PCT conditions for respiratory specimen processing before mycobacterium detection by culture or molecular testing.
通过常规程序检测和鉴定分枝杆菌属的抗酸杆菌需要从患者标本中培养生物体,然后测试分离株的各种表型特征。这些方法可能需要几天到 1 个月或更长时间。开发了一些用于测试抗酸杆菌生长培养物的高度特异性分子探针,大大缩短了某些分枝杆菌分离株的鉴定时间。最近,聚合酶链反应和等温核酸扩增技术已用于检测,为临床样本中的结核分枝杆菌检测提供高度特异性和合理的灵敏度。拥有能够检测多种分枝杆菌物种同时排除来自临床样本中常见的其他细菌的交叉反应信号的扩增测定系统将是有用的。已经成功地使用一种检测版本进行了实验,该检测版本可同时检测六种常见的临床分离分枝杆菌物种的核酸扩增产物的存在或不存在。 N.I.H. 之间的联合专利申请贝克曼库尔特公司最近已提交。由于样品制备是分子诊断测定的关键组成部分,因此我们发起了一项新的努力来研究压力循环技术 (PCT) 在提高分枝杆菌细胞核酸释放效率方面的有效性。最近,根据 CRADA 与 BBI Biotech 合作,将气压循环器(压力循环装置)引入 CPD。在核酸提取之前使用 PCT 作为预处理,我们能够增加含有戈登分枝杆菌的悬浮液和结核分枝杆菌悬浮液的平均 PCR 信号。约翰霍普金斯大学结核病研究中心和 BBI 正在启动一项前瞻性合作研究,以评估在通过培养或分子测试检测分枝杆菌之前,不同 PCT 条件在呼吸道标本处理中的效用。

项目成果

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Steven h FISCHER其他文献

Steven h FISCHER的其他文献

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{{ truncateString('Steven h FISCHER', 18)}}的其他基金

Making Worcester Safe for our Children by 2010: Lead Poisoning Awareness & Prev
到 2010 年让伍斯特对我们的孩子来说是安全的:铅中毒意识
  • 批准号:
    7383587
  • 财政年份:
    2007
  • 资助金额:
    --
  • 项目类别:
Evaluation Of Real-time Pcr Assay For Diagnosis Of Pcp U
实时 PCR 检测诊断 Pcp U 的评估
  • 批准号:
    6825576
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Development Of A Polymerase Chain Reaction Procedure For
聚合酶链反应程序的开发
  • 批准号:
    6825445
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Development of a Polymerase Chain Reaction Procedure for Quantitative Measurement
用于定量测量的聚合酶链式反应程序的开发
  • 批准号:
    6103699
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Evaluation Of A Real-time PCR Assay For Diagnosis Of PCP Using Oral Washes
使用口腔洗液诊断 PCP 的实时 PCR 检测的评估
  • 批准号:
    7593109
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Evaluation Of Real-time Pcr Assay For Diagnosis Of Pcp
实时 PCR 检测对 PCP 诊断的评估
  • 批准号:
    7004811
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Detection and Identification of Mycobacteria in Clinical
临床分枝杆菌的检测与鉴定
  • 批准号:
    7004941
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Cytomegalovirus: Quantitative Polymerase Chain Reaction
巨细胞病毒:定量聚合酶链反应
  • 批准号:
    6542076
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
DETECTION AND IDENTIFICATION OF MYCOBACTERIA IN CLINICAL SPECIMENS
临床标本中分枝杆菌的检测和鉴定
  • 批准号:
    6289476
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
Development Of A Polymerase Chain Reaction Procedure For
聚合酶链反应程序的开发
  • 批准号:
    6675195
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:

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宿主诱导基因编辑(Hi-GE)的开发:细菌性疾病控制的新平台
  • 批准号:
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  • 财政年份:
    2019
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The relationship between malarial anaemia, neutrophil function and susceptibility to invasive bacterial disease.
疟疾贫血、中性粒细胞功能与侵袭性细菌性疾病易感性之间的关系。
  • 批准号:
    MR/P000959/2
  • 财政年份:
    2017
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    --
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    Research Grant
Redefining the role of autophagy in bacterial disease
重新定义自噬在细菌性疾病中的作用
  • 批准号:
    10519116
  • 财政年份:
    2016
  • 资助金额:
    --
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Redefining the role of autophagy in bacterial disease
重新定义自噬在细菌性疾病中的作用
  • 批准号:
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Redefining the role of autophagy in bacterial disease
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    10764559
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The relationship between malarial anaemia, neutrophil function and susceptibility to invasive bacterial disease.
疟疾贫血、中性粒细胞功能与侵袭性细菌性疾病易感性之间的关系。
  • 批准号:
    MR/P000959/1
  • 财政年份:
    2016
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Redefining the role of autophagy in bacterial disease
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The treatment of bacterial disease of plants by bacteriophage coated nanoparticles
噬菌体包被纳米颗粒治疗植物细菌性疾病
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    BB/K501979/1
  • 财政年份:
    2013
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    Training Grant
Wintertime Seasonality of Influenza and Invasive Bacterial Disease: Influence of Environment, Pathogen Interactions, Time Scales, and Geography
流感和侵袭性细菌性疾病的冬季季节性:环境、病原体相互作用、时间尺度和地理的影响
  • 批准号:
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    Operating Grants
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