Evaluation Of A Real-time PCR Assay For Diagnosis Of PCP Using Oral Washes

使用口腔洗液诊断 PCP 的实时 PCR 检测的评估

• 批准号: 7593109
• 负责人: Steven h FISCHER
• 金额: $ 0.5万
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7593109
• 关键词: Acid Fast Bacillae Staining Method; Alveolar; Biological Assay; California; Clinic; Clinical; Collaborations; Detection; Diagnosis; Disease; Evaluation; Exposure to; Fluorescent Antibody Technique; General Hospitals; Genomics; Giemsa stain; Health Personnel; Health Professional; Hospitals; Immunocompromised Host; Incidence; Individual; Infection; Irrigation; Lung diseases; Medical; Methods; Microscopic; Mycobacterium Infections; Oral; Patients; Pneumocystis; Pneumocystis jiroveci; Pneumonia; Polymerase Chain Reaction; Population; Process; Respiratory Tract Infections; Sampling; San Francisco; Sodium Glutamate; Specimen; Sputum; Staining method; Stains; Standards of Weights and Measures; System; Testing; Time; Tuberculosis; Uganda; United States National Institutes of Health; Universities; base; cytarabine/methotrexate protocol; design; pathogen; respiratory

Pneumocystis jiroveci is an important cause of pneumonia (PCP) in immunocompromised individuals. The standard approach for diagnosing PCP is a microscopic examination of smears prepared from induced sputum or bronchial alveolar lavage (BAL) samples. We have also developed a rapid, quantitative PCR (polymerase chain reaction) assay for the detection of P. jiroveci in respiratory samples. Because the PCR assay is more sensitive that microscopic examination, we have demonstrated the P. jiroveci can be detected in noninvasive specimens such as oral washes. Additionally, the test can detect patient who are only colonized or have sub-clinical infections. A collaborative study with the University of California at San Francisco (UCSF) is underway to attempt to detect P. jiroveci colonization of the upper airways of healthcare workers after exposure to patients with PCP. Oral wash specimens have been collected from 60 healthcare workers and will be processed by PCR for P. jiroveci. The results of this study will define the incidence of asymptomatic colonization with this important pathogen. A second study has been initiated with collaborators at San Francisco General Hospital and Mulago Hospital workers in Uganda. Oral washes have been collected from patients with respiratory diseases who present with respiratory diseases at the hospital Tuberculosis Clinic. These specimens have been processed for mycobacterial infections (i.e., acid-fast smear and culture of the specimen) A new study was begun in collaboration with the San Francisco General Hospital, NIH Clinical Center CCM coworkers, and Mulago Hospital workers in Uganda to collect specimens from patients with respiratory diseases to determine if Pneumocystis jiroveci can be detected in oral washes. Specimens have been sent to the NIH for studies using the MSG Pneumocystis PCR system. Over 100 samples have been received to date.

吉罗维氏肺孢子虫是免疫功能低下者肺炎(PCP)的重要原因。诊断PCP的标准方法是对诱导痰或支气管肺泡灌洗(BAL)样本制备的涂片进行显微镜检查。我们还开发了一种快速、定量的聚合酶链式反应(聚合酶链式反应)分析方法，用于检测呼吸道样本中的支原体。因为聚合酶链式反应检测比显微镜检查更敏感，我们已经证明在非侵入性的标本中可以检测到杰罗韦氏肺孢子虫，例如口腔洗涤剂。此外，该检测还可以检测到仅被殖民或有亚临床感染的患者。 一项与加州大学旧金山分校(UCSF)的合作研究正在进行中，试图检测在接触PCP患者后医护人员上呼吸道中的P.jirovei定殖率。已从60名医护人员那里收集了口腔拭子样本，并将通过聚合酶链式反应(PCR)对吉罗韦克肺炎支原体进行处理。这项研究的结果将确定这种重要病原体无症状定植的发生率。 与旧金山总医院的合作者和乌干达穆拉戈医院的工作人员一起启动了第二项研究。从医院结核病诊所出现呼吸道疾病的呼吸系统疾病患者那里收集了口腔洗液。这些标本已经过分枝杆菌感染的处理(即标本的抗酸涂片和培养)。 与旧金山总医院、美国国立卫生研究院临床中心CCM同事和乌干达穆拉戈医院的工作人员合作开始了一项新的研究，以收集呼吸道疾病患者的样本，以确定口腔洗涤剂中是否可以检测到吉罗维氏肺孢子虫。标本已被送往美国国立卫生研究院，使用味精肺孢子虫聚合酶链式反应系统进行研究。到目前为止，已经收到了100多个样品。