Fluorescent Activated Cell Sorter for C. elegans (worms)

用于秀丽隐杆线虫（蠕虫）的荧光激活细胞分选仪

• 批准号: 6738620
• 负责人: Susan E Mango
• 金额: $ 13.4万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6738620
• 关键词: Caenorhabditis elegans; biomedical equipment purchase; cell sorting; flow cytometry

DESCRIPTION (provided by applicant): The nematode Caenorhabditis elegans has become an important experimental system used to study a wide variety of biological problems germane to eucaryotes. The size, limited cell number and approachable genetics has made C. elegans a highly useful organism. This proposal is to purchase a Union Biometrica Biosort 2. This instrument will both analyze and sort C. elegans on the basis of size and fluorescence; it is a "cell sorter for worms". The high capacity of the instrument in combination with its ability to discriminate worms of different sizes or expressing different fluorescent proteins permits genetic screens on a scale that was previously unknown. The capacity of the instrument to sort living worms (50,000 worms/hour) will provide enough transgenic or mutant animals to permit biochemical studies. The instrument will be used by seven different principal investigators, who belong to five different departments housed in three different colleges (College of Arts and Sciences, College of Pharmacy, School of Medicine). The instrument will be housed in our existing Fluorescence Activated Cell Sorter Facility. This core facility is centrally located on the University of Utah campus and is proximal to all of the investigators. This facility has been in operation for over ten years and is overseen by a full-time Ph.D. director and a skilled technician. The director provides training and support to users using the current Fluorescence activated cell sorters and scanners and will do so for the union Biometrica Biosort 2. There is strong institutional support for this instrument. The institution will provide the salary of the Director and the technician. In addition to space in the core facility the institution will guarantee the maintenance of the instrument. This institutional commitment will ensure that the instrument can be used by investigators without having to charge prohibitive hourly rates. The instrument's use would be monitored by an oversight committee that would manage issues dealing with priorities and publicity. The head of this committee will be a member of the Core Facilities Oversight Board. This administrative structure will provide a seamless management of the "worm sorter" within the framework of institutional Core Facilities. In summary, a fluorescent activated cell sorter for C. elegans would provide new capabilities that would greatly benefit the strong group of C. elegans researchers at the University of Utah. It would both facilitate existing research projects and permit the development of new projects that could not function without this novel instrument.

描述（由申请人提供）：线虫秀丽隐杆线虫已成为用于研究与真核生物密切相关的各种生物学问题的重要实验系统。由于其体积小、细胞数量有限、遗传学上的可接近性，使C.线虫是一种非常有用的生物。本提案旨在购买Union Biometrica Biosort 2。该仪器将对C进行分析和排序。根据大小和荧光，它是一种“蠕虫细胞分选器”。该仪器的高容量与其区分不同大小的蠕虫或表达不同荧光蛋白的能力相结合，允许在以前未知的规模上进行遗传筛选。该仪器分选活蠕虫的能力（50，000条蠕虫/小时）将提供足够的转基因或突变动物，以进行生物化学研究。 该仪器将由七个不同的主要研究者使用，他们属于三个不同学院（艺术与科学学院，药学院，医学院）的五个不同部门。该仪器将被安置在我们现有的荧光激活细胞分选设备中。该核心设施位于犹他州大学校园的中心，靠近所有研究人员。该设施已运营十多年，由一名全职博士监督。导演和一个熟练的技术人员。该主管为使用当前荧光激活细胞分选仪和扫描仪的用户提供培训和支持，并将为联合生物计量生物分类2提供培训和支持。 该文书得到了强有力的机构支持。该机构将提供主任和技术员的工资。除了核心设施的空间外，该机构还将保证仪器的维护。这一机构承诺将确保调查人员可以使用该工具，而不必收取令人望而却步的小时费率。该文书的使用将由一个监督委员会监督，该委员会将管理与优先事项和宣传有关的问题。该委员会的负责人将是核心设施监督委员会的成员。这一行政结构将在机构核心设施的框架内对“蠕虫分类器”进行无缝管理。 综上所述，建立了一种用于C. elegans将提供新的能力，这将大大有利于C的强大群体。犹他州大学的线虫研究人员说。它既可以促进现有的研究项目，也可以开发没有这一新工具就无法运作的新项目。