Mark I Model 68A Rodent/Cell line Gamma Irradiator

描述（由申请人提供）：本申请的目的是申请资金购买Mark I型号68A啮齿动物铯（137Cs）辐照器，供几个小公共卫生资助的科学家使用，这些科学家需要这种专用设备来照射小鼠或细胞系，作为他们研究的关键组成部分。这些科学家对转基因小鼠的辐照是在免疫能力小鼠中进行骨髓移植的先决条件，研究的目的是：1。研究特定骨髓细胞群对动脉粥样硬化或心肌梗死病变发展进程的贡献2。研究各种粘附分子在动脉粥样硬化和心肌梗死中炎症细胞正常运输和迁移中的生物力学贡献，其中双或三重基因敲除通常对小鼠是致命的。3. 目的：引入胸腺前体，在引入辐照宿主后，在发育中的胸腺细胞中删除编码c-myb的基因。这样做是为了更有效地测试c-myb在胸腺阳性和阴性选择中的作用，而不是将目标c-myb位点与必要的Cre转基因一起培育到各种小鼠背景4。探讨克隆群体t淋巴细胞在流感和呼吸道合胞病毒感染小鼠模型中诱导肺损伤的作用以可重复的方式诱导肠粘膜损伤，以研究各种细胞因子和生长因子在粘膜炎症和修复中的作用。在以下类型的研究中，细胞系的辐照是必要的：生产用于维持培养的饲养细胞，如胚胎干细胞。对细胞系诱导特定的DNA损伤，以研究各种治疗方法的致敏效应，以诱导辐射敏感性，以开发新的癌症治疗策略。该辐照器将取代1984年购买的现有装置Nordion Gamma cell 40，该装置的修复部件已不再市售。

DESCRIPTION (provided by applicant): The purpose of this submission is to request funding to purchase a Mark I Model 68A rodent cesium (137Cs) irradiator for use by several PHS funded scientists that require this item of specialized equipment to irradiate mice or cell lines as a critical component of their research. The irradiation of transgenic mice by these scientists is a pre-requisite to bone marrow transplantation in immune competent mice when the intent of the research is to: 1. Study the contribution of specific bone marrow cells populations to the progression of lesion development in atherosclerosis or myocardial infarction 2. Study the biomechanical contribution of various adhesion molecules in the normal trafficking and migration of inflammatory cells in atherosclerosis and myocardial infarction where the double or triple gene knockouts would normally be lethal to the mice. 3. To introduce thymic precursors that engineered to delete the gene encoding c-myb in developing thymocytes after introduction into irradiated hosts. This is done in order to test the role of c-myb during positive and negative selection in the thymus more efficiently than breeding the targeted c-myb locus, along with the necessary Cre transgenes onto a variety of mouse backgrounds 4. Investigate the contribution of clonal populations T-lymphocytes to the induction of pulmonary damage in mouse models of influenza and respiratory syncytial virus infection 5. Induce damage to the intestinal mucosa in a manner that is reproducible to investigate the role of various cytokines and growth factors in mucosal inflammation and repair. The irradiation of cell lines is necessary in the following types of studies: 1. Produce feeder cells for maintaining cultures a fastidious cell lines such as embryonic stem cells 2. Induce defined DNA damage to cell lines to study the sensitizing effects of various treatments to induce radiation sensitivity for the development of novel cancer therapeutic strategies This irradiator will replace an existing unit Nordion Gamma Cell 40 purchased in 1984 and for which repair parts are no longer commercially available.