An 800 MHz NMR spectrometer for the Boston area

描述(由申请人提供)：申请资助一台新的18.79T(800 MHz 1H)核磁共振(核磁共振)光谱仪，安装在布兰迪斯大学，供波士顿和新英格兰生物医学研究团体使用。该光谱仪将主要用于高分辨率、高灵敏度的多维核磁共振实验，通常用于溶液或流动双相中同位素标记的(15N、13C、2H)生物样品。理想情况下，该光谱仪将配备四个射频(RF)通道、一个1 H高频通道和三个宽带RF通道，用于三重共振和2H去耦合脉冲序列，在所有通道上都具有脉冲整形和波形产生能力。所要求的光谱仪将包括三轴脉冲场梯度能力，以及在0.1℃内稳定的样品温度调节。需要三个探头，包括配备x，y，z梯度的标准三共振1H探测探头，用于直接探测其他核的宽带探头，以及配备梯度的氦冷却三共振1H探测低温探头，以提高1H观测实验的信噪比。 这种仪器是与波士顿地区的高场核磁共振用户合作寻找的。尽管该地区有密集的生物医学研究活动，但该地区还没有通用的高场核磁共振设施，其光谱仪可以满足现代生物医学核磁共振谱的技术要求。Brandeis大学是操作和维护该仪器的合理选择，因为该大学拥有维护和操作该仪器的技术技能，而且该大学有提供使用维护良好且使用方便的最先进核磁共振设施的历史。

DESCRIPTION (provided by applicant): Funding is requested for a new 18.79 T (800 MHz 1H) nuclear magnetic resonance (NMR) spectrometer to be sited at Brandeis University for use by the Boston and New England biomedical research community. This spectrometer will be used primarily for high-resolution, high-sensitivity multidimensional NMR experiments, typically with isotopically labeled (15N, 13C, 2H) biological samples in solution or mobile biphases. The spectrometer will ideally be equipped with four radiofrequency (RF) channels, one high band RF channel for 1 H, and three broadband RF channels for use in triple resonance and 2H-decoupling pulse sequences, with pulse-shaping and wave-form generating capabilities on all channels. Three axis pulsed-field gradient capabilities will be included in the requested spectrometer, as well as sample temperature regulation stable within 0.1 C. Three probes are requested, including a standard triple-resonance 1 H-detection probe equipped with x,y,z gradients, a broad band probe for direct detection of other nuclei, and a He-cooled triple-resonance 1 H-detection cryoprobe equipped with gradients for improved signal-to-noise in 1 H observe experiments. This instrument is sought in collaboration with high-field NMR users throughout the Boston area. Despite the intensive biomedical research activity in the region, there is no general access high field NMR facility in the area with spectrometers that can meet the technical demands of modern biomedical NMR spectroscopy. A case will be made that Brandeis University is a logical choice for operating and maintaining this instrument, since the technical skills are available to maintain and operate the instrument, and the University has a history of providing access to state-of-the-art NMR facilities that are well-maintained and convenient to use.