An 800 MHz NMR spectrometer for the Boston area

描述（由申请人提供）：申请资助一台新的18.79 T （800 MHz 1H）核磁共振（NMR）光谱仪，该光谱仪将位于布兰代斯大学，供波士顿和新英格兰生物医学研究界使用。该光谱仪将主要用于高分辨率、高灵敏度的多维核磁共振实验，通常用于同位素标记（15N、13C、2H）的溶液或流动两相生物样品。理想情况下，该光谱仪将配备四个射频（RF）通道，一个用于1 H的高频段RF通道和三个用于三重共振和2H去耦脉冲序列的宽带RF通道，并在所有通道上具有脉冲整形和波形生成能力。要求的光谱仪将包括三轴脉冲场梯度能力，以及在0.1 c内稳定的样品温度调节。要求三个探针，包括配备x，y，z梯度的标准三共振1 H探测探针，用于直接探测其他核的宽带探针，以及配备梯度的氦冷却三共振1 H探测低温探针，用于改善1 H观察实验中的信噪比。

DESCRIPTION (provided by applicant): Funding is requested for a new 18.79 T (800 MHz 1H) nuclear magnetic resonance (NMR) spectrometer to be sited at Brandeis University for use by the Boston and New England biomedical research community. This spectrometer will be used primarily for high-resolution, high-sensitivity multidimensional NMR experiments, typically with isotopically labeled (15N, 13C, 2H) biological samples in solution or mobile biphases. The spectrometer will ideally be equipped with four radiofrequency (RF) channels, one high band RF channel for 1 H, and three broadband RF channels for use in triple resonance and 2H-decoupling pulse sequences, with pulse-shaping and wave-form generating capabilities on all channels. Three axis pulsed-field gradient capabilities will be included in the requested spectrometer, as well as sample temperature regulation stable within 0.1 C. Three probes are requested, including a standard triple-resonance 1 H-detection probe equipped with x,y,z gradients, a broad band probe for direct detection of other nuclei, and a He-cooled triple-resonance 1 H-detection cryoprobe equipped with gradients for improved signal-to-noise in 1 H observe experiments. This instrument is sought in collaboration with high-field NMR users throughout the Boston area. Despite the intensive biomedical research activity in the region, there is no general access high field NMR facility in the area with spectrometers that can meet the technical demands of modern biomedical NMR spectroscopy. A case will be made that Brandeis University is a logical choice for operating and maintaining this instrument, since the technical skills are available to maintain and operate the instrument, and the University has a history of providing access to state-of-the-art NMR facilities that are well-maintained and convenient to use.