An 800 MHz NMR spectrometer for the Boston area

描述（由申请人提供）：申请资金用于新的18.79 T（800 MHz 1H）核磁共振（NMR）光谱仪，该光谱仪将位于布兰迪斯大学，供波士顿和新英格兰生物医学研究界使用。该光谱仪将主要用于高分辨率、高灵敏度的多维NMR实验，通常使用溶液或移动的两相中的同位素标记的（15 N，13 C，2 H）生物样品。理想情况下，光谱仪将配备四个射频（RF）通道，一个用于1 H的高频带RF通道，以及三个用于三重谐振和2 H去耦脉冲序列的宽带RF通道，所有通道都具有脉冲整形和波形生成功能。三轴脉冲场梯度能力将包括在所要求的光谱仪中，以及样品温度调节稳定在0.1 C内。需要三种探针，包括配备有x、y、z梯度的标准三重共振1H-检测探针，用于直接检测其他核的宽带探针，以及配备有梯度的He冷却三重共振1H-检测冷冻探针，用于改善1H观察实验中的信噪比。 该仪器是寻求与高场核磁共振用户在整个波士顿地区的合作。尽管该地区开展了密集的生物医学研究活动，但该地区没有普遍使用的高场核磁共振设施，其光谱仪可以满足现代生物医学核磁共振光谱学的技术要求。一个案件将作出布兰代斯大学是一个合乎逻辑的选择操作和维护这个仪器，因为技术技能可用于维护和操作仪器，和大学有提供访问的历史国家的最先进的NMR设施，维护良好，方便使用。

DESCRIPTION (provided by applicant): Funding is requested for a new 18.79 T (800 MHz 1H) nuclear magnetic resonance (NMR) spectrometer to be sited at Brandeis University for use by the Boston and New England biomedical research community. This spectrometer will be used primarily for high-resolution, high-sensitivity multidimensional NMR experiments, typically with isotopically labeled (15N, 13C, 2H) biological samples in solution or mobile biphases. The spectrometer will ideally be equipped with four radiofrequency (RF) channels, one high band RF channel for 1 H, and three broadband RF channels for use in triple resonance and 2H-decoupling pulse sequences, with pulse-shaping and wave-form generating capabilities on all channels. Three axis pulsed-field gradient capabilities will be included in the requested spectrometer, as well as sample temperature regulation stable within 0.1 C. Three probes are requested, including a standard triple-resonance 1 H-detection probe equipped with x,y,z gradients, a broad band probe for direct detection of other nuclei, and a He-cooled triple-resonance 1 H-detection cryoprobe equipped with gradients for improved signal-to-noise in 1 H observe experiments. This instrument is sought in collaboration with high-field NMR users throughout the Boston area. Despite the intensive biomedical research activity in the region, there is no general access high field NMR facility in the area with spectrometers that can meet the technical demands of modern biomedical NMR spectroscopy. A case will be made that Brandeis University is a logical choice for operating and maintaining this instrument, since the technical skills are available to maintain and operate the instrument, and the University has a history of providing access to state-of-the-art NMR facilities that are well-maintained and convenient to use.