Zeiss LSM 510-NLO multiphoton laser scanning microscope

描述（由申请人提供）：奥尔巴尼医学院（AMC）的一组研究人员正在申请资金购买Zeiss LSM 510-NLO多光子激光扫描显微镜（MPLSM），这是AMC无法使用的仪器。赖斯博士正在分析正常和病理皮肤的皮肤神经支配，以研究带状疱疹引起的疼痛和神经功能障碍，使用多荧光标记的人类皮肤厚样本。奥唐纳博士的工作解决了间隙连接在神经同步化中的作用，方法是从丘脑核中的一群神经元进行同步双全细胞记录，然后在厚脑切片中观察荧光黄填充的细胞，结合免疫荧光，这是一种独特的方法，结合细胞可视化和电生理学，以更好地了解间隙连接功能的调节。Kimelberg博士的亚细胞定位研究涉及使用MPLSM对厚海马切片中记录和染料填充的星形胶质细胞进行双重或三重免疫标记，以研究缺血中兴奋性氨基酸释放的机制。Temple博士的研究重点是了解中枢神经系统（CNS）发育过程中不同细胞类型的产生。她将使用延时MPLSM成像来跟踪皮质心室板活组织切片中干细胞的分裂，并结合免疫荧光来识别这些干细胞不对称分裂的后代。Teitler博士提出，抗精神病药物的反向激动剂活性可以通过研究组成型激活突变体（CAM）人氯氮平敏感性5-HT 2A，5-HT 2d，5-HT 6和5-HT 7受体来揭示。他将使用CAM人5 HT受体/GFP融合蛋白结合延时MPLSM成像来检查抗精神病药物对这些受体向膜的运输和受体下调期间的影响。Herrick-Davis博士的目标是确定调节5-HT 2C受体激活的分子机制，以了解5-HT 2C受体在正常和疾病状态下的功能。特别是，她在活细胞中使用FRET来确定突变受体是否与天然受体物理结合或二聚化，从而形成非功能性受体复合物。Banas博士将使用MPLSM来研究由口服链球菌制成的成熟活生物膜中基因表达变化引起的生理学。该小组将充分利用蔡司LSM 510-NLO的能力。

DESCRIPTION (provided by applicant): A group of investigators at the Albany Medical College (AMC) is requesting funds to purchase a Zeiss LSM510-NLO multiphoton laser scanning microscope (MPLSM), an instrument that is not available for their use at AMC. Dr. Rice is analyzing cutaneous innervation in normal and pathologic skin to study pain and neural dysfunction resulting from Herpes Zoster using mutiply-fluorescent-labeled thick samples of human skin. Dr. O'Donnell's work addresses the role of gap junctions in neural synchronizations by performing simultaneous dual whole-cell recordings from a cluster of neurons in the nucleus accumbens and then visualizing the lucifer yellow filled cells coupled with immunofluorescence in thick brain slices, a unique approach that combines cell visualization and electrophysiology to get a better idea of the modulation of gap junction function. Dr. Kimelberg's subcellular localization studies involve double or triple immunolabelling of recorded and dye-filled astrocytes in thick hippocampal sections using MPLSM to study mechanisms of excitatory amino-acid release in ischemia. Dr. Temple's research studies focus on understanding the generation of diverse cell types during development of the central nervous system (CNS). She will use time-lapse MPLSM imaging to follow the division of stem cells in live tissue slices of the cortical ventricular plate coupled with immunofluorescence to identify the progeny of asymetric division of these stem cells. Dr. Teitler proposes that the inverse agonist activity of antipsychotic drugs can be revealed by studying constitutively activated mutant (CAM) Human clozapine-sensitive 5-HT2A, 5-HT2d, 5-HT6, and 5-HT7 receptors. He will use CAM human 5HT receptor/GFP fusion proteins coupled with time-lapse MPLSM imaging to examine the effect antipsychotic drugs have on the trafficking of these receptors to the membrane and during receptor down-regulation. Dr. Herrick-Davis' goal is to identify molecular mechanisms that regulate 5-HT2C receptor activation to understand how 5-HT2C receptors function in normal and disease states. In particular she uses FRET in live cells to determine if mutant receptors physically associate or dimerize with native receptors, and thus form non-functional receptor complexes. Dr. Banas will use MPLSM to study the physiology that results from changes in gene expression in mature, live biofilms made by oral Steprococci. This group will fully utilize the capabilities of the Zeiss LSM510-NLO.