Zeiss LSM 510-NLO multiphoton laser scanning microscope

描述（由申请人提供）：奥尔巴尼医学院 (AMC) 的一组研究人员正在申请资金购买蔡司 LSM510-NLO 多光子激光扫描显微镜 (MPLSM)，但 AMC 无法使用这种仪器。赖斯博士正在分析正常和病理皮肤的皮肤神经支配，以使用多重荧光标记的厚厚人类皮肤样本来研究带状疱疹引起的疼痛和神经功能障碍。 O'Donnell 博士的工作解决了间隙连接在神经同步中的作用，方法是对伏隔核中的一组神经元进行同步双全细胞记录，然后在厚脑切片中观察荧光黄填充的细胞，并结合免疫荧光，这是一种结合细胞可视化和电生理学的独特方法，可以更好地了解间隙连接功能的调节。 Kimelberg 博士的亚细胞定位研究涉及使用 MPLSM 对海马厚切片中记录的和充满染料的星形胶质细胞进行双重或三重免疫标记，以研究缺血中兴奋性氨基酸释放的机制。 Temple 博士的研究重点是了解中枢神经系统 (CNS) 发育过程中不同细胞类型的生成。她将使用延时 MPLSM 成像来跟踪皮质心室板活组织切片中干细胞的分裂，并结合免疫荧光来识别这些干细胞不对称分裂的后代。 Teitler 博士提出，通过研究组成型激活突变体 (CAM) 人类氯氮平敏感 5-HT2A、5-HT2d、5-HT6 和 5-HT7 受体，可以揭示抗精神病药物的反向激动剂活性。他将使用 CAM 人类 5HT 受体/GFP 融合蛋白与延时 MPLSM 成像相结合，检查抗精神病药物对这些受体转运到膜上以及受体下调期间的影响。 Herrick-Davis 博士的目标是确定调节 5-HT2C 受体激活的分子机制，以了解 5-HT2C 受体在正常和疾病状态下如何发挥作用。特别是，她在活细胞中使用 FRET 来确定突变受体是否与天然受体发生物理关联或二聚化，从而形成非功能性受体复合物。 Banas 博士将使用 MPLSM 研究由口腔链球菌产生的成熟活生物膜中基因表达变化引起的生理学。该小组将充分利用蔡司 LSM510-NLO 的功能。

DESCRIPTION (provided by applicant): A group of investigators at the Albany Medical College (AMC) is requesting funds to purchase a Zeiss LSM510-NLO multiphoton laser scanning microscope (MPLSM), an instrument that is not available for their use at AMC. Dr. Rice is analyzing cutaneous innervation in normal and pathologic skin to study pain and neural dysfunction resulting from Herpes Zoster using mutiply-fluorescent-labeled thick samples of human skin. Dr. O'Donnell's work addresses the role of gap junctions in neural synchronizations by performing simultaneous dual whole-cell recordings from a cluster of neurons in the nucleus accumbens and then visualizing the lucifer yellow filled cells coupled with immunofluorescence in thick brain slices, a unique approach that combines cell visualization and electrophysiology to get a better idea of the modulation of gap junction function. Dr. Kimelberg's subcellular localization studies involve double or triple immunolabelling of recorded and dye-filled astrocytes in thick hippocampal sections using MPLSM to study mechanisms of excitatory amino-acid release in ischemia. Dr. Temple's research studies focus on understanding the generation of diverse cell types during development of the central nervous system (CNS). She will use time-lapse MPLSM imaging to follow the division of stem cells in live tissue slices of the cortical ventricular plate coupled with immunofluorescence to identify the progeny of asymetric division of these stem cells. Dr. Teitler proposes that the inverse agonist activity of antipsychotic drugs can be revealed by studying constitutively activated mutant (CAM) Human clozapine-sensitive 5-HT2A, 5-HT2d, 5-HT6, and 5-HT7 receptors. He will use CAM human 5HT receptor/GFP fusion proteins coupled with time-lapse MPLSM imaging to examine the effect antipsychotic drugs have on the trafficking of these receptors to the membrane and during receptor down-regulation. Dr. Herrick-Davis' goal is to identify molecular mechanisms that regulate 5-HT2C receptor activation to understand how 5-HT2C receptors function in normal and disease states. In particular she uses FRET in live cells to determine if mutant receptors physically associate or dimerize with native receptors, and thus form non-functional receptor complexes. Dr. Banas will use MPLSM to study the physiology that results from changes in gene expression in mature, live biofilms made by oral Steprococci. This group will fully utilize the capabilities of the Zeiss LSM510-NLO.