NORAN OZ CONFOCAL LASER SCANNING SYSTEM

NORAN OZ 共焦激光扫描系统

• 批准号: 2776171
• 负责人: Joseph E Mazurkiewicz
• 金额: $ 24.86万
• 依托单位: ALBANY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2776171
• 关键词: bioimaging /biomedical imaging; biomedical equipment; biomedical equipment purchase; confocal scanning microscopy

A group of investigators at the Albany Medical College (AMC) are requesting funds to purchase a Noran OZ Confocal Laser Scanning Microscope (CLSM), a type of instrument heretofore unavailable for their use at the AMC. Drs. McKeown-Longo, LaFlamme, Higgins, Mazurkiewicz, and Saba, are studying cell-cell and cell-matrix interactions. The studies focus on various signaling molecules, integrins, cell junctions, cytoskeleton, and matrix proteins and the use of immunofluorescence and the CLSM to localize these molecules or elements of the cell structures to note their distribution with relationship to one another, to cell junctions (tight junctions, adherens junctions, and hemi-desmosomes) or cell-matrix interactions at integrin-cytoskeleton-matrix proteins, subsequent to a variety of stimuli, developmental cues, or gene transfections. Dr. Rice is analyzing fluorescently labelled structures and molecules in the complex three-dimensional organization of the cutaneous endings during development of the whisker pads of mice and rats. Dr. Black uses the fluorescent long-chain fatty acid analog, BODIPY-3823, to assess the uptake of fatty acids by Fat1p, a membrane- bound long-chain fatty acid transport protein found in yeast plasma membranes. He is studying the effects of mutations in the gene, deltafat1, on fatty acid transport. Dr. Schneider is testing a novel hypothesis of secretion in adrenal chromaffin cells that involves a Na- Ca exchange protein that is present in secretory granule membranes and that is inserted into the plasma membrane during exocytosis. Dr. Temple, in studying differentiation in primary cultures of rat cortical neurons, examines the appearance and activity of various receptor linked Ca++ channels in her cells. Both researchers are monitoring physiologic responses in cytoplasmic [Ca++] with msec kinetics that require the fast-scan capabilities of the Noran, as does Dr. Melendez who is studying the effect of MnSOD overexpression on the oxidant status of tumorigenic and non-tumorigenic cells in live cultures. Drs. Gosselin and Mazurkiewicz study the trafficking of GFP tagged-MHC Class II (MHC II) and exogenously derived antigens in antigen presentation in live human monocytes in culture. This group of researches will fully utilize the capabilities of the Noran OZ CLSM.

奥尔巴尼医学院（AMC）的一组研究人员正在申请资金购买一台Noran OZ共聚焦激光扫描显微镜（CLSM），这是一种迄今为止AMC无法使用的仪器。 McKeown-Longo、LaFlamme、Higgins、Mazurkiewicz和Saba博士正在研究细胞与细胞和细胞与基质的相互作用。 这些研究集中于各种信号分子、整合素、细胞连接、细胞骨架和基质蛋白，以及使用免疫荧光和CLSM来定位这些分子或细胞结构的元件，以注意它们与彼此、与细胞连接的关系的分布（紧密连接、粘附连接和半桥粒）或整合素-细胞骨架-基质蛋白的细胞-基质相互作用，随后的各种刺激，发育线索，或基因转染。 赖斯博士正在分析小鼠和大鼠胡须垫发育过程中皮肤末梢复杂三维组织中的荧光标记结构和分子。 Black博士使用荧光长链脂肪酸类似物BODIPY-3823来评估Fat 1 p对脂肪酸的吸收，Fat 1 p是一种在酵母质膜中发现的膜结合长链脂肪酸转运蛋白。 他正在研究deltafat 1基因突变对脂肪酸转运的影响。 Schneider博士正在测试一种肾上腺嗜铬细胞分泌的新假设，该假设涉及存在于分泌颗粒膜中的Na- Ca交换蛋白，并在胞吐过程中插入质膜。 Temple博士在研究大鼠皮层神经元原代培养物的分化时，检查了她细胞中各种受体相关Ca++通道的外观和活性。 两位研究人员都在监测细胞质[Ca++]中的生理反应，需要Noran的快速扫描能力，Melendez博士也在研究MnSOD过表达对活培养物中致瘤性和非致瘤性细胞氧化状态的影响。 Gosselin和Mazurkiewicz博士研究了培养的活人类单核细胞中抗原递呈中GFP标记的MHC II类（MHC II）和外源性抗原的运输。 这组研究将充分利用Noran OZ CLSM的功能。