Laser Scanning Confocal Microscope

描述（由申请人提供）：本申请是为了在NCRR共享仪器资助计划的赞助下购买奥林巴斯Fluoview 500激光扫描共聚焦显微镜。Olympus FV 500是一款激光扫描共聚焦显微镜，具有多达5个独立的检测通道、可独立调节的共聚焦针孔、全自动滤光片和反射镜选择以及直观而强大的软件包。所要求的三种激光器，氩、红色氦氖和绿色氦氖，将为大多数应用提供激发信号，未来可能会扩展使用其他激光器。这项申请得到了七名NIH资助的研究人员和一名其他来源资助的研究人员的支持。乔治敦大学医学中心（GUMC）非常需要额外的共聚焦显微镜，以补充隆巴迪癌症中心的现有仪器，并扩大校园研究人员的能力。目前的共享工具一直是以最大能力订阅的。目前可用的仪器足以满足大多数用途，但该应用中的许多研究者需要所需仪器提供的更多功能。这个应用程序是由一组研究人员提出的，他们需要定期使用共聚焦显微镜，他们熟悉它的使用，其中许多人已经看到他们的研究计划因Lombardi共聚焦的有限可用性而受到限制。新的共聚焦将被安置和维护在细胞生物学成像核心中，该核心还将容纳透射电子显微镜，激光捕获显微切割显微镜和宽视场光和荧光显微镜以及相关配件。成像核心将由指定的技术人员运行，并由PI与适当的监督委员会共同监督。所有合格的研究者都可以按服务收费使用该仪器，本申请中的研究者将获得优先权。支持继续维护和运行该仪器的资金将由GUMC通过研究院院长办公室提供。该申请的成功资助将填补GUMC校园共聚焦显微镜设施扩展的明确需求，并将允许从NIH资助的研究中获得的结果的质量和数量的增加。

DESCRIPTION (provided by applicant): This application is for funds to purchase an Olympus Fluoview 500 laser scanning confocal microscope under the auspices of the NCRR Shared Instrumentation Grant program. The Olympus FV500 is a laser scanning confocal microscope with up to 5 independent channels of detection, independently adjustable confocal pinholes, fully automated filter and mirror selection, and an intuitive and powerful software package. The three lasers requested, argon, red Helium-Neon and green Helium-Neon, will provide excitatory signals for most applications, with the expansion with additional lasers possible in the future. This application is supported by seven NIH funded investigators and one investigator funded from other sources. There is a tremendous need for an additional confocal microscope at Georgetown University Medical Center (GUMC) to complement the current instrument at the Lombardi Cancer Center and expand the capabilities of researchers on the campus. The current shared instrument is consistently subscribed to maximum capacity. The currently available instrument is adequate for most uses but many of the investigators in this application require further capabilities that will be provided by the requested instrument. This application is put forward by a group of investigators who require the use of confocal microscopy on a regular basis, who are familiar with its use, and many of whom have seen their research programs curtailed by the limited availability of the Lombardi cofocal. The new confocal will be housed and maintained in a Cell Biology imaging core, which will also house a transmission electron microscope, laser capture microdissection microscope and wide field light and fluorescent microscopes and associated accessories. The imaging core will be run by a technician who is to be named, and overseen by the PI in conjunction with appropriate oversight committees. The instrument will be available to all qualified investigators on a fee for service basis, with investigators in this application receiving priority. Funds to support the continued maintenance and operation of this instrument are to be provide by GUMC through the Office of the Dean of Research. Successful funding of this application will fill a clear need for expansion of confocal microscopy facilities on the GUMC campus and will allow for an increase in the quality and quantity of results derived from NIH funded research.