Laser Scanning Confocal Microscope

描述(由申请者提供)：本申请是为了筹集资金购买奥林巴斯FluoView 500激光扫描共聚焦显微镜，该显微镜由NCRR共享仪器资助计划资助。奥林巴斯FV500是一款激光扫描共聚焦显微镜，具有多达5个独立的检测通道、独立可调的共焦针孔、全自动滤镜和镜片选择以及直观而强大的软件包。所要求的三个激光器，Ar，红色氦-霓虹灯和绿色氦-霓虹灯，将为大多数应用提供激发信号，未来可能会扩大更多的激光器。这项申请得到了七名NIH资助的调查人员和一名其他来源资助的调查人员的支持。乔治城大学医学中心(GUMC)非常需要一台额外的共焦显微镜，以补充隆巴迪癌症中心现有的仪器，并扩大校园内研究人员的能力。目前的共享仪器一直被预订到最大容量。目前可用的仪器对大多数用途来说是足够的，但这一申请中的许多调查员需要所请求的仪器将提供的更多能力。这项申请是由一群研究人员提出的，他们需要定期使用共焦显微镜，他们熟悉它的使用，其中许多人发现他们的研究计划因Lombardi共焦显微镜的有限而受到限制。新的共焦显微镜将安装在细胞生物学成像核心中并进行维护，该核心还将容纳一个透射电子显微镜、激光捕获显微解剖显微镜和广视场光学和荧光显微镜以及相关附件。成像核心将由一名将被点名的技术人员运行，并由PI与适当的监督委员会一起监督。该仪器将以服务费的形式向所有合格的调查人员提供，这项申请的调查人员将优先获得。GUMC将通过研究主任办公室提供资金，以支持该仪器的持续维护和运行。这项申请的成功资助将满足GUMC园区扩展共焦显微镜设施的明显需求，并将使NIH资助的研究成果的质量和数量得到提高。

DESCRIPTION (provided by applicant): This application is for funds to purchase an Olympus Fluoview 500 laser scanning confocal microscope under the auspices of the NCRR Shared Instrumentation Grant program. The Olympus FV500 is a laser scanning confocal microscope with up to 5 independent channels of detection, independently adjustable confocal pinholes, fully automated filter and mirror selection, and an intuitive and powerful software package. The three lasers requested, argon, red Helium-Neon and green Helium-Neon, will provide excitatory signals for most applications, with the expansion with additional lasers possible in the future. This application is supported by seven NIH funded investigators and one investigator funded from other sources. There is a tremendous need for an additional confocal microscope at Georgetown University Medical Center (GUMC) to complement the current instrument at the Lombardi Cancer Center and expand the capabilities of researchers on the campus. The current shared instrument is consistently subscribed to maximum capacity. The currently available instrument is adequate for most uses but many of the investigators in this application require further capabilities that will be provided by the requested instrument. This application is put forward by a group of investigators who require the use of confocal microscopy on a regular basis, who are familiar with its use, and many of whom have seen their research programs curtailed by the limited availability of the Lombardi cofocal. The new confocal will be housed and maintained in a Cell Biology imaging core, which will also house a transmission electron microscope, laser capture microdissection microscope and wide field light and fluorescent microscopes and associated accessories. The imaging core will be run by a technician who is to be named, and overseen by the PI in conjunction with appropriate oversight committees. The instrument will be available to all qualified investigators on a fee for service basis, with investigators in this application receiving priority. Funds to support the continued maintenance and operation of this instrument are to be provide by GUMC through the Office of the Dean of Research. Successful funding of this application will fill a clear need for expansion of confocal microscopy facilities on the GUMC campus and will allow for an increase in the quality and quantity of results derived from NIH funded research.