Acquisition of Cryogenically-Cooled NMR Probe at 800 MHz

采集 800 MHz 低温冷却 NMR 探头

• 批准号: 6580518
• 负责人: RONALD A VENTERS
• 金额: $ 25.05万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580518
• 关键词: biomedical equipment purchase; cryoscience; electron probe spectrometry; nuclear magnetic resonance spectroscopy; structural biology

DESCRIPTION (provided by applicant): Duke University will purchase a cryogenically-cooled, ultra-high sensitivity triple resonance, 1H{13C115N}, 5 mm probe and associated closed-cycle cryogenic system for the Varian Inova 800 MHz nuclear magnetic resonance (NMR) system currently operating in its NMR Center. Cryogenically cooling an NMR probe and the associated pre-amplifier hardware has been demonstrated to provide a significant improvement in achievable signal-to-noise ratios in studies involving both samples of biologically important macromolecules at millimolar and micromolar concentrations and of small molecule metabolites at nanomolar concentrations. Developments in NMR instrumentation, NMR experimentation and molecular biology have provided researchers with the ability to investigate more challenging and, by extension, more biomedically relevant systems involving large monomers and complexes, macromolecules with limited solubility, biomolecules under conditions that mimic those encountered in-vivo, metabolites at very low concentrations and the dynamics of systems over a wide range of timescales. Currently, the 800 MHz system, located in the Duke University NMR Center, is utilized by a large contingent of scientists, both within the Duke community and across the Southeast region, to address a broad range of biologically significant projects. Seven investigators in Biochemistry and the NMR Center at Duke, along with nine scientists from the University of North Carolina, North Carolina State University, the University of Virginia, the NIEHS and St. Jude Children's Hospital constitute the initial majors users of the cryogenically-cooled probe. All of these investigators are currently involved in technically demanding research aimed at elucidating the structure, function and dynamics of important biomedical and biological macromolecules. The need for even higher sensitivity than currently offered by conventional probes has been expressed by many of these groups as they extend their research to more technically demanding problems. The addition of an ultra-high sensitivity cryogenically cooled probe to the 800 MHz spectrometer in the Duke NMR Center represents a major advance to a unique resource in the region: an addition that will enhance an already vibrant research community.

描述（由申请人提供）：杜克大学将为目前在其NMR中心运行的Varian Inova 800 MHz核磁共振（NMR）系统购买一个低温冷却、超高灵敏度三重共振、1H{13 C115 N}、5 mm探头和相关闭合循环低温系统。低温冷却的NMR探针和相关的前置放大器硬件已被证明提供了一个显着的改善，在可实现的信噪比的研究中，涉及在毫摩尔和微摩尔浓度的生物学重要的大分子和小分子代谢物在纳摩尔浓度的样品。核磁共振仪器，核磁共振实验和分子生物学的发展为研究人员提供了研究更具挑战性的能力，并且通过扩展，涉及大单体和复合物，溶解度有限的大分子，模拟体内遇到的生物分子条件下的生物分子，非常低浓度的代谢物和系统动力学的生物医学相关系统在很宽的时间范围内。目前，位于杜克大学NMR中心的800 MHz系统被杜克社区和东南地区的大量科学家使用，以解决广泛的生物学重要项目。杜克生物化学和核磁共振中心的七名研究人员、沿着来自北卡罗来纳州大学、北卡罗来纳州州立大学、弗吉尼亚大学、NIEHS和圣裘德儿童医院的九名科学家构成了低温冷却探头的最初主要用户。所有这些研究人员目前都参与了技术要求很高的研究，旨在阐明重要的生物医学和生物大分子的结构，功能和动力学。这些研究小组中的许多人已经表达了对比传统探头更高灵敏度的需求，因为他们将研究扩展到技术要求更高的问题。在杜克NMR中心的800 MHz光谱仪上增加了一个超高灵敏度的低温冷却探头，这是该地区独特资源的一个重大进步：这一增加将增强本已充满活力的研究社区。