Fluorescence Lifetime and Steady-State Spectrometer

描述(申请人提供)：核心用户组需要一台荧光分光光度计来确定荧光寿命，测量时间分辨的荧光各向异性，获取稳态激发和发射光谱，并确定稳态荧光偏振。我们需要一台光子技术国际(PTI)TimeMaster 720T型T格式荧光寿命仪，带有包括偏振附件和温度控制在内的稳态荧光测量子系统附件。该仪器将用于评估结构和动力学实验所需的荧光标记蛋白质和核苷酸，通过荧光共振能量转移测量分子距离，测量荧光蛋白质和指示性染料在溶液和细胞中的旋转动力学，以及测量活细胞分泌颗粒内的pH变化。核心用户群体由来自医学院内三个不同部门的六个独立的NIH资助的实验室组成。研究领域包括化学机械偶联、细胞运动和肌肉收缩的机制和调节、兴奋-分泌偶联、药物设计、离子转运和核糖体功能。核心用户群体在光谱设备的操作、设计和维护方面进行了广泛的培训。

DESCRIPTION (provided by applicant): A core user group requires a spectrofluorometer to determine fluorescence lifetimes, measure time resolved fluorescence anisotropy, acquire steady-state excitation and emission spectra, and determine steady-state fluorescence polarization. We request a Photon Technology International (PTI) TimeMaster Model 720T T-format fluorescence lifetime instrument with a steady-state fluorometry subsystem add-on which includes polarization accessories and temperature control. The instrument will be used to evaluate fluorescently labeled proteins and nucleotides required for structural and kinetic experiments, measure molecular distances by fluorescence resonance energy transfer, measure rotational dynamics of fluorescent proteins and indicator dyes in solution and in cells, and measure pH changes within the secretory granules of live cells. The core user group comprises six independent NIH-funded laboratories from three different departments within the medical school. The fields of investigation include chemomechanical coupling, mechanisms and regulation of cell motility and muscle contraction, excitation-secretion coupling, drug design, ion transport, and ribosome function. The core user group has extensive training in the operation, design, and maintenance of spectroscopic equipment.