Confocal Laser Scanning Microscope

描述（由申请人提供）：本提案的目的是更换显微镜服务实验室（MSL）中已有14年历史的Zeiss LSM 210共聚焦激光扫描显微镜（CLSM），该实验室是病理学和实验室医学系的核心设施。在过去的14年里，这种共焦显微镜在许多研究领域的使用有了巨大的增长。去年，LSM 210被来自25个部门的51名主要研究人员的109名科学家使用。这种仪器被广泛使用，但它已经过时得无可救药了，而且无法取代。它不再得到蔡司的支持（即使是零部件），它使用古老的计算机技术，并且有灾难性故障的风险。这个核心设施中的新CLSM将大大扩展研究人员的研究能力。研究人员现在使用多种荧光标记;许多研究需要在含水样品中进行亚细胞成像;一次数据收集可能需要数百张图像。使用声光可调滤波器（AOTF）控制激发光束强度，三个超灵敏光电倍增管（PMT）检测器中的每一个的可变孔用于控制发射强度和共焦分辨率，极其灵活的光束扫描控制允许用户定义感兴趣的区域，丰富的激光激发线选择，和上级计算机技术联合收割机结合在新的CLSM中，生产出一种仪器，将在未来为研究人员服务。MSL的工作人员都是显微镜成像技术方面的专家，并擅长作为服务设施。他们将接受一个内部咨询委员会的指导。MSL得到医学院的大力支持，占地2000平方米。英尺位于医疗大楼中心的一个空间。该设施中的一个新的CLSM将使研究人员最容易获得这项新技术，从而使该中心的医学研究界受益。这是一个几乎完全修改的重新申请。由于我们的校园正在购买两个新的多光子CLSM，我们已经删除了我们的请求的多光子部分，我们已经修改了主要用户组。项目说明中增加了大量的初步数据和公布的数据。

DESCRIPTION (provided by applicant): The purpose of this proposal is to replace a 14-year-old Zeiss LSM 210 confocal laser scanning microscope (CLSM) in the Microscopy Services Laboratory (MSL), a core facility in the Department of Pathology and Laboratory Medicine. The past 14 years have seen tremendous growth in the use of this confocal microscope in numerous areas of research. Last year, the LSM 210 was used by 109 scientists working under 51 principal investigators from 25 departments. This instrument is used extensively, but it is hopelessly out of date and is not upgradeable. It is no longer supported by Zeiss (even for spare parts), it uses ancient computer technology, and it is at risk of catastrophic failure. A new CLSM in this core facility will greatly expand research capabilities of investigators. Researchers now use multiple fluorescent labels; many studies require subcellular imaging deep within an aqueous mounted sample; several hundred images may be required for a single data collection run. The use of acustico optical tunable filters (AOTF) to control excitation beam intensity, a variable )inhole for each of the three super sensitive photo-multiplier tube (PMT) detectors for control of emission intensity and confocal resolution, extremely flexible beam scanning control allowing user defined regions of interest, a rich selection of laser excitation lines, and superior computer technology combine in the new CLSM to produce an instrument that will serve researchers at UNC well into the future. Members of the MSL staff are experts in microscope imaging technology and are skilled in functioning as a service facility. They will be guided by an internal advisory committee. The MSL is well supported by the School of Medicine and occupies 2000 sq. ft. of space centrally located in the medical complex. A new CLSM in this facility will benefit the medical research community at UNC by making this new technology most accessible to investigators. This is a re-application that is almost completely revised. Because two new Multi-Photon CLSMs are being purchased on our campus, we have removed the multi-photon portion of our request and we have modified the major user group. Substantial preliminary and published data have been added to project descriptions.