Confocal Laser Scanning Microscope

描述（由申请人提供）：本提案的目的是替换显微镜服务实验室（MSL）中已有14年历史的蔡司LSM 210共聚焦激光扫描显微镜（CLSM），该实验室是病理学和检验医学系的核心设施。在过去的14年里，这种共聚焦显微镜在许多研究领域的应用取得了巨大的增长。去年，lsm210被来自25个部门的51名首席研究员的109名科学家使用。这种仪器被广泛使用，但它已经过时，无法升级。它不再得到蔡司的支持（即使是备件），它使用的是古老的计算机技术，有发生灾难性故障的风险。一个新的CLSM在这个核心设施将大大扩大研究人员的研究能力。研究人员现在使用多种荧光标记；许多研究需要亚细胞成像深入水装样品；单个数据收集运行可能需要数百个图像。使用声学光学可调滤光片（AOTF）来控制激发光束强度，三个超灵敏的光倍增管（PMT）探测器中的每一个用于控制发射强度和共聚焦分辨率，极其灵活的光束扫描控制，允许用户定义感兴趣的区域，丰富的激光激发线选择，和先进的计算机技术相结合，在新的CLSM产生的仪器，将为北卡罗来纳大学的研究人员在未来很好。MSL的工作人员都是显微镜成像技术方面的专家，在服务设施方面也很熟练。他们将由一个内部咨询委员会指导。医学院有医学院配套设施，占地面积2000平方米。6英尺的空间位于医疗综合体的中心。该设施的新CLSM将使研究人员最容易获得这项新技术，从而使北卡罗来纳大学的医学研究界受益。这是一个几乎完全修改过的重新申请。因为两个新的多光子clsm正在我们的校园购买，我们已经删除了我们的请求的多光子部分，我们已经修改了主要用户组。项目描述中增加了大量初步和已发表的数据。

DESCRIPTION (provided by applicant): The purpose of this proposal is to replace a 14-year-old Zeiss LSM 210 confocal laser scanning microscope (CLSM) in the Microscopy Services Laboratory (MSL), a core facility in the Department of Pathology and Laboratory Medicine. The past 14 years have seen tremendous growth in the use of this confocal microscope in numerous areas of research. Last year, the LSM 210 was used by 109 scientists working under 51 principal investigators from 25 departments. This instrument is used extensively, but it is hopelessly out of date and is not upgradeable. It is no longer supported by Zeiss (even for spare parts), it uses ancient computer technology, and it is at risk of catastrophic failure. A new CLSM in this core facility will greatly expand research capabilities of investigators. Researchers now use multiple fluorescent labels; many studies require subcellular imaging deep within an aqueous mounted sample; several hundred images may be required for a single data collection run. The use of acustico optical tunable filters (AOTF) to control excitation beam intensity, a variable )inhole for each of the three super sensitive photo-multiplier tube (PMT) detectors for control of emission intensity and confocal resolution, extremely flexible beam scanning control allowing user defined regions of interest, a rich selection of laser excitation lines, and superior computer technology combine in the new CLSM to produce an instrument that will serve researchers at UNC well into the future. Members of the MSL staff are experts in microscope imaging technology and are skilled in functioning as a service facility. They will be guided by an internal advisory committee. The MSL is well supported by the School of Medicine and occupies 2000 sq. ft. of space centrally located in the medical complex. A new CLSM in this facility will benefit the medical research community at UNC by making this new technology most accessible to investigators. This is a re-application that is almost completely revised. Because two new Multi-Photon CLSMs are being purchased on our campus, we have removed the multi-photon portion of our request and we have modified the major user group. Substantial preliminary and published data have been added to project descriptions.