Confocal Laser Scanning Microscope

描述(由申请人提供)：这项建议的目的是在显微镜服务实验室(MSL)取代已有14年历史的蔡司LSM 210共焦激光扫描显微镜(MSL)，该实验室是病理和实验室医学系的核心设施。在过去的14年里，这种共聚焦显微镜在许多研究领域的使用都有了巨大的增长。去年，LSM 210被109名科学家使用，他们在来自25个部门的51名首席调查人员手下工作。这种仪器被广泛使用，但它已经过时了，而且不能升级。它不再得到蔡司的支持(即使是备件)，它使用的是古老的计算机技术，它面临着灾难性故障的风险。这一核心设施中的一个新的CLSM将极大地扩展调查人员的研究能力。研究人员现在使用多个荧光标记；许多研究需要在水中安装样本的深处进行亚细胞成像；一次数据收集可能需要数百张图像。使用声光可调滤光器(AOTF)来控制激发光束强度，三个超灵敏光电倍增管(PMT)探测器用于控制发射强度和共焦分辨率的可变内孔，允许用户定义感兴趣区域的极其灵活的光束扫描控制，丰富的激光激发线选择，以及先进的计算机技术在新的CLSM中相结合，生产出一种将在未来很好地为北卡罗来纳大学的研究人员服务的仪器。MSL的工作人员都是显微镜成像技术方面的专家，并熟练地发挥着服务设施的作用。他们将由一个内部咨询委员会指导。MSL得到了医学院的大力支持，占地2000平方米。英国《金融时报》位于医疗综合体中心的空间。该设施中的一个新的CLSM将使这项新技术更容易为研究人员所用，从而使北卡罗来纳大学的医学研究社区受益。这是一个几乎完全修改过的重新申请。由于我们的园区将购买两台新的多光子CLSM，因此我们删除了请求中的多光子部分，并修改了主要用户组。在项目说明中增加了大量的初步数据和已公布的数据。

DESCRIPTION (provided by applicant): The purpose of this proposal is to replace a 14-year-old Zeiss LSM 210 confocal laser scanning microscope (CLSM) in the Microscopy Services Laboratory (MSL), a core facility in the Department of Pathology and Laboratory Medicine. The past 14 years have seen tremendous growth in the use of this confocal microscope in numerous areas of research. Last year, the LSM 210 was used by 109 scientists working under 51 principal investigators from 25 departments. This instrument is used extensively, but it is hopelessly out of date and is not upgradeable. It is no longer supported by Zeiss (even for spare parts), it uses ancient computer technology, and it is at risk of catastrophic failure. A new CLSM in this core facility will greatly expand research capabilities of investigators. Researchers now use multiple fluorescent labels; many studies require subcellular imaging deep within an aqueous mounted sample; several hundred images may be required for a single data collection run. The use of acustico optical tunable filters (AOTF) to control excitation beam intensity, a variable )inhole for each of the three super sensitive photo-multiplier tube (PMT) detectors for control of emission intensity and confocal resolution, extremely flexible beam scanning control allowing user defined regions of interest, a rich selection of laser excitation lines, and superior computer technology combine in the new CLSM to produce an instrument that will serve researchers at UNC well into the future. Members of the MSL staff are experts in microscope imaging technology and are skilled in functioning as a service facility. They will be guided by an internal advisory committee. The MSL is well supported by the School of Medicine and occupies 2000 sq. ft. of space centrally located in the medical complex. A new CLSM in this facility will benefit the medical research community at UNC by making this new technology most accessible to investigators. This is a re-application that is almost completely revised. Because two new Multi-Photon CLSMs are being purchased on our campus, we have removed the multi-photon portion of our request and we have modified the major user group. Substantial preliminary and published data have been added to project descriptions.