Ultra High-Speed Imaging System

描述（由申请人提供）： 该共享仪器拨款申请的目的是购买 Imacon 200 超高速成像系统，由杜克大学 NIH 资助的一组研究人员共享。主要用户组由来自 3 个不同 NIH 机构的 7 R01 和 1 P01 拨款支持。这些研究人员（来自机械工程和材料科学、泌尿外科、细胞生物学、放射肿瘤学和生物医学工程系）迫切需要高速成像分析，以便有效推进他们的研究。将立即受益于该拟议仪器的项目涵盖了广泛的主题，从治疗肾结石疾病的冲击波碎石术（SWL）到SWL中的伤口愈合，到脂质体药物载体的设计，到果蝇生殖系干细胞分裂的调节，到用于癌症治疗的基因治疗。 Imacon 200 提供独特的高速成像系统，利用多个增强型 CCD 模块来提供同步取景和条纹记录，每个序列中最多可录制 16 帧高分辨率图像。每个增强型 CCD 模块都可以电子选通，曝光时间从 100 毫秒到 5 纳秒，从而提供从 10 帧/秒到 2 亿帧/秒的有效帧速率。 Imacon 200 系统的操作由专用计算机通过光纤链路进行远程控制，以实现可靠的图像捕获和定量数据提取。杜克大学目前不存在能够执行任何这些功能的类似设备。因此，该系统对于进行高速光弹性和阴影成像以更好地了解冲击波-石头相互作用、血管中的腔内气泡动力学、气泡破裂和相关微流在脂质体载体触发释放中的作用、微射流促进的果蝇胚胎基因递送、热疗介导的基因递送和激活、电穿孔过程中跨细胞质膜和核膜的DNA转运以及肿瘤微循环中红细胞的流变学至关重要。超高速成像核心设施的建立将极大地增强这些研究项目以及这些研究人员和杜克大学其他研究人员的科学贡献。

DESCRIPTION (provided by applicant): The purpose of this shared instrumentation grant application is to acquire an Imacon 200 ultra high-speed imaging system to be shared by a group of NIH funded investigators at Duke University. The Major Users Group is supported by 7 R01 and 1 P01 grants from 3 different NIH institutes. These investigators (from the Depts. of Mechanical Engineering and Materials Science, Urologic Surgery, Cell Biology, Radiation Oncology, and Biomedical Engineering) have critical needs for high-speed imaging analyses in order to effectively advance their research. The projects that will immediately benefit from this proposed instrument cover a wide range of topics, from shock wave lithotripsy (SWL) for the treatment of kidney stone disease, to wound healing in SWL, to the design of liposomal drug carriers, to regulation of germ line stem cell division in Drosophila, to gene therapy for cancer treatment. The Imacon 200 offers a unique high-speed imaging system utilizing multiples of intensified CCD modules to provide simultaneous framing and streak recording, up to 16 frames of high-resolution images in each sequence. Each intensified CCD module can be gated electronically with an exposure time from 100 ms down to 5 ns, thus providing an effective framing rate from 10 frames/second to 200 million frames/second. The operation of the Imacon 200 system is controlled remotely via fiber optical link by a dedicated computer for reliable image capture and quantitative data extraction. No comparable equipment capable of performing any of these functions currently exists at Duke University. This system, therefore, is critical for performing high-speed photoelastic and shadowgraph imaging to better understand shock wave-stone interaction, intraluminal bubble dynamics in blood vessels, the role of bubble collapse and associated microstreaming in triggered release of liposomal carriers, microjet-faciliated gene delivery to Drosophila embryos, hyperthermia-mediated gene delivery and activation, DNA transport across the plasma and nuclei membranes of the cells during electroporation, and rheology of red blood cells in tumor microcirculation. The establishment of a core facility in ultra high-speed imaging will greatly enhance these research programs and the scientific contributions by these and other investigators at Duke University.