Ultra High-Speed Imaging System

描述（由申请人提供）： 本共享仪器拨款申请的目的是获得一个Imacon 200超高速成像系统，供杜克大学的一组NIH资助的研究人员共享。主要用户组由来自3个不同NIH研究所的7个R 01和1个P01赠款支持。这些调查人员（来自各部门）。机械工程和材料科学、泌尿外科、细胞生物学、放射肿瘤学和生物医学工程）对高速成像分析有着迫切的需求，以便有效地推进他们的研究。将立即受益于这一拟议的仪器的项目涵盖了广泛的主题，从冲击波碎石术（SWL）治疗肾结石疾病，伤口愈合的SWL，脂质体药物载体的设计，调节果蝇生殖系干细胞分裂，基因治疗癌症治疗。Imacon 200提供了一个独特的高速成像系统，利用多个增强型CCD模块提供同步成帧和条纹记录，每个序列最多可记录16帧高分辨率图像。每个增强型CCD模块都可以电子选通，曝光时间从100 ms到5 ns，从而提供从10帧/秒到2亿帧/秒的有效帧速率。Imacon 200系统的操作由专用计算机通过光纤链路远程控制，以进行可靠的图像捕获和定量数据提取。杜克大学目前没有能够执行任何这些功能的类似设备。因此，该系统对于进行高速光弹性和阴影成像以更好地理解冲击波-结石相互作用、血管中的管腔内气泡动力学、气泡塌陷和相关微流在脂质体载体的触发释放中的作用、微射流促进的基因递送至果蝇胚胎、高血压介导的基因递送和激活、电穿孔过程中DNA穿过细胞质膜和核膜的转运，以及肿瘤微循环中红细胞的流变学。超高速成像核心设施的建立将极大地增强这些研究项目以及杜克大学这些和其他研究人员的科学贡献。

DESCRIPTION (provided by applicant): The purpose of this shared instrumentation grant application is to acquire an Imacon 200 ultra high-speed imaging system to be shared by a group of NIH funded investigators at Duke University. The Major Users Group is supported by 7 R01 and 1 P01 grants from 3 different NIH institutes. These investigators (from the Depts. of Mechanical Engineering and Materials Science, Urologic Surgery, Cell Biology, Radiation Oncology, and Biomedical Engineering) have critical needs for high-speed imaging analyses in order to effectively advance their research. The projects that will immediately benefit from this proposed instrument cover a wide range of topics, from shock wave lithotripsy (SWL) for the treatment of kidney stone disease, to wound healing in SWL, to the design of liposomal drug carriers, to regulation of germ line stem cell division in Drosophila, to gene therapy for cancer treatment. The Imacon 200 offers a unique high-speed imaging system utilizing multiples of intensified CCD modules to provide simultaneous framing and streak recording, up to 16 frames of high-resolution images in each sequence. Each intensified CCD module can be gated electronically with an exposure time from 100 ms down to 5 ns, thus providing an effective framing rate from 10 frames/second to 200 million frames/second. The operation of the Imacon 200 system is controlled remotely via fiber optical link by a dedicated computer for reliable image capture and quantitative data extraction. No comparable equipment capable of performing any of these functions currently exists at Duke University. This system, therefore, is critical for performing high-speed photoelastic and shadowgraph imaging to better understand shock wave-stone interaction, intraluminal bubble dynamics in blood vessels, the role of bubble collapse and associated microstreaming in triggered release of liposomal carriers, microjet-faciliated gene delivery to Drosophila embryos, hyperthermia-mediated gene delivery and activation, DNA transport across the plasma and nuclei membranes of the cells during electroporation, and rheology of red blood cells in tumor microcirculation. The establishment of a core facility in ultra high-speed imaging will greatly enhance these research programs and the scientific contributions by these and other investigators at Duke University.