Ultra High-Speed Imaging System

描述(由申请人提供)： 这项共享仪器拨款申请的目的是获得一台IMACON 200超高速成像系统，由杜克大学的一组NIH资助的研究人员共享。主要用户组得到了来自3个不同NIH研究所的7个R01和1个P01赠款的支持。这些调查员(来自部门。机械工程和材料科学、泌尿外科、细胞生物学、放射肿瘤学和生物医学工程等专业的研究人员对高速成像分析有着迫切的需求，以便有效地推进他们的研究。将立即受益于这一拟议仪器的项目涵盖了广泛的主题，从用于治疗肾结石疾病的冲击波碎石术(SWL)，到SWL的伤口愈合，到脂质体药物载体的设计，到对果蝇生殖系干细胞分裂的调控，再到用于癌症治疗的基因治疗。IMACON 200提供独特的高速成像系统，利用多个增强型CCD模块同时提供成帧和条纹记录，每个序列最多16帧高分辨率图像。每个增强的CCD模块可以电子选通，曝光时间从100ms到5 ns，从而提供从10帧/秒到2亿帧/秒的有效成帧速率。IMACON 200系统的运行由一台专用计算机通过光纤链路远程控制，以实现可靠的图像捕获和定量数据提取。杜克大学目前还没有能够执行上述任何功能的同类设备。因此，该系统对于进行高速光弹成像和阴影成像是至关重要的，以更好地了解冲击波-结石相互作用、血管内气泡动力学、气泡破裂和相关的微流在脂质体载体触发释放中的作用、微射流促进的基因传递到果蝇胚胎、高温介导的基因传递和激活、电穿孔过程中DNA跨细胞质膜和核膜的传输以及肿瘤微循环中红细胞的流变学。超高速成像核心设施的建立将极大地加强这些研究计划以及杜克大学这些研究人员和其他研究人员的科学贡献。

DESCRIPTION (provided by applicant): The purpose of this shared instrumentation grant application is to acquire an Imacon 200 ultra high-speed imaging system to be shared by a group of NIH funded investigators at Duke University. The Major Users Group is supported by 7 R01 and 1 P01 grants from 3 different NIH institutes. These investigators (from the Depts. of Mechanical Engineering and Materials Science, Urologic Surgery, Cell Biology, Radiation Oncology, and Biomedical Engineering) have critical needs for high-speed imaging analyses in order to effectively advance their research. The projects that will immediately benefit from this proposed instrument cover a wide range of topics, from shock wave lithotripsy (SWL) for the treatment of kidney stone disease, to wound healing in SWL, to the design of liposomal drug carriers, to regulation of germ line stem cell division in Drosophila, to gene therapy for cancer treatment. The Imacon 200 offers a unique high-speed imaging system utilizing multiples of intensified CCD modules to provide simultaneous framing and streak recording, up to 16 frames of high-resolution images in each sequence. Each intensified CCD module can be gated electronically with an exposure time from 100 ms down to 5 ns, thus providing an effective framing rate from 10 frames/second to 200 million frames/second. The operation of the Imacon 200 system is controlled remotely via fiber optical link by a dedicated computer for reliable image capture and quantitative data extraction. No comparable equipment capable of performing any of these functions currently exists at Duke University. This system, therefore, is critical for performing high-speed photoelastic and shadowgraph imaging to better understand shock wave-stone interaction, intraluminal bubble dynamics in blood vessels, the role of bubble collapse and associated microstreaming in triggered release of liposomal carriers, microjet-faciliated gene delivery to Drosophila embryos, hyperthermia-mediated gene delivery and activation, DNA transport across the plasma and nuclei membranes of the cells during electroporation, and rheology of red blood cells in tumor microcirculation. The establishment of a core facility in ultra high-speed imaging will greatly enhance these research programs and the scientific contributions by these and other investigators at Duke University.