IMMUNOHISTOCHEMISTRY SHARED RESOURCE

免疫组织化学共享资源（ISR）是CCSG的新项目，但在另一个VUMC研究中心的支持下，它的服务已经提供了近十年。许多癌症研究者使用这个资源，它提供小鼠组织和/或细胞切片的免疫组织化学染色。众所周知，为了利用近年来出现的大量抗体，需要制定标准化的免疫方案。ISR集中了这项任务，防止了不必要的重复，并提供了获得国家认证的人员和最先进的设备。简而言之，ISR通过维持相关标记物（抗血清）的库存来支持癌症相关研究；免疫组化抗血清筛选/优化；标准化

The Immunohistochemistry Shared Resource (ISR) is new to the CCSG, but its services have been available for nearly a decade under the auspices of another VUMC research center. Many cancer investigators use this Resource, which offers immunohistochemical staining in mouse tissue and/or cellular sections. It is well recognized that the ability to make use of the large number of antibodies that have become available in Irecent years requires development of standardized immunoprotocols. The ISR centralizes this task and prevents needless repetition, and gives access to nationally-certified personnel and state-of-the-art equipment. In brief, the ISR supports cancer-related research by maintaining stocks of relevant markers (antisera) with ready-to-go protocols; screening/optimizing antisera for immunohistochemistry; standardizing quality assurance; and providing immunostaining of tissue microarrays. It will support state-of-the-art embedding, sectioning, and staining for microscopic specimens by providing paraffin embedding; frozen embedding and sectioning; sections suitable for routine staining, immunostaining, in situ hybridization, laser capture dissention and for RNA extraction from archival blocks; and the provision of guidance in techniques of quantitative morphometric analysis. Dr. Lillian B. Nanney is the Director of the Immunohistochemistry Shared Resource. She brings to this venture over 20 years of research and teaching experience in microscopy, histology and cell biology. She is supported by a lab manager and other technicians who are readily available to VICC investigators.