Morphology Core

形态核心的主要目标是提高两位新手的多功能性和生产力 皮肤研究人员(P&F资助的科学家)和成熟的皮肤研究人员，通过促进访问 针对皮肤样本的显微分析技术。我们将继续实现这些目标 通过作为成功/失败免疫组织化学染色方案的储存库，证明皮肤特异性 抗血清，通过为免疫组化染色提供新的抗血清的筛选和优化， 并在调查人员试图确定皮肤异常和反应时协助他们进行解释。至 为了实现这些目标，核心将提供最先进的嵌入、切片(冰冻或石蜡)，以及 由训练有素的专家及时、低成本地进行染色(主要是免疫组织化学染色)。这个 主任和核心人员将在对皮肤有用的显微技术方面提供教学帮助和建议 对包括P&F调查员、国家发改委资助的调查员和他们的博士后在内的广泛个人的分析 研究员、研究生、学生和实验室人员。形态核心将继续提供 最初尝试尖端免疫组织化学技术的先锋/培育环境 在经验丰富的团队的指导下取得成功。Core还将继续推出新的试剂 以及表现出更大敏感性的技术进步。我们将继续创造工作机会 使用途径特异性免疫探针的方案。核心将密切合作，帮助调查人员定制 适用于组织微阵列的标准化免疫标记物面板。相互之间的协作互动 其他SDRCC核心将轻松实现。由于这个核心是范德比尔特机构核心系统的一部分， 与其他相关核心建立了良好的联系，如人类组织和病理学共享 资源，分子识别(抗血清生产)核心，小动物成像核心，电子 显微核心、蛋白质组学核心或细胞成像/共聚焦核心。

The primary objective of the Morphology Core is to enhance the versatility and productivity for both novice cutaneous investigators (P & F funded scientists) and established skin investigators by facilitating access to techniques directed toward the microscopic analysis of skin samples. We will continue to accomplish these goals by serving as a repository of successful/unsuccessful immunohistochemical staining protocols with proven skinspecific antisera, by providing screening and optimization of new antisera for immunohistochemical staining, and by assisting investigators with interpretation as they seek to define skin abnormalities and responses. To achieve these objectives, the Core will render state-of-the-art embedding, sectioning (frozens or paraffins), and staining (primarily immunohistochemical staining) in a timely and low-cost basis by trained experts. The Director and Core personnel will supply teaching assistance and advice in microscopic techniques useful for skin analysis for a wide range of individuals including P & F investigators, funded SDRCC investigators, and their postdoctoral fellows, graduate, students, and laboratory personnel. The Morphology Core will continue to provide a pioneering/nurturing environment where cutting edge immunohistochemical techniques are initially tried and achieved under the guidance of an experienced team. The Core will also continue to introduce of new reagents and technological advancements that exhibit greater sensitivities. We will continue to generate working protocols with pathway specific immunoprobes. The Core will work closely to help investigators customize panels of standardized immunomarkers suitable for use in tissue microarrays. Cooperative interactions among the other SDRCC Cores will occur with ease. Since this Core is a portion of Vanderbilt's Institutional Core system, liaisons are well established with other relevant Cores such as the Human Tissue and Pathology Shared Resource, Molecular Recognition (Antisera Production) Core, Small Animal Imaging Core, the Electron Microscopy Core, Proteomics Core or Cell Imaging/Confocal Core.