Morphology Core

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The primary objective of the Morphology Core is to enhance the versatility and productivity for both novice cutaneous investigators (P & F funded scientists) and established skin investigators by facilitating access to techniques directed toward the microscopic analysis of skin samples. We will continue to accomplish these goals by serving as a repository of successful/unsuccessful immunohistochemical staining protocols with proven skinspecific antisera, by providing screening and optimization of new antisera for immunohistochemical staining, and by assisting investigators with interpretation as they seek to define skin abnormalities and responses. To achieve these objectives, the Core will render state-of-the-art embedding, sectioning (frozens or paraffins), and staining (primarily immunohistochemical staining) in a timely and low-cost basis by trained experts. The Director and Core personnel will supply teaching assistance and advice in microscopic techniques useful for skin analysis for a wide range of individuals including P & F investigators, funded SDRCC investigators, and their postdoctoral fellows, graduate, students, and laboratory personnel. The Morphology Core will continue to provide a pioneering/nurturing environment where cutting edge immunohistochemical techniques are initially tried and achieved under the guidance of an experienced team. The Core will also continue to introduce of new reagents and technological advancements that exhibit greater sensitivities. We will continue to generate working protocols with pathway specific immunoprobes. The Core will work closely to help investigators customize panels of standardized immunomarkers suitable for use in tissue microarrays. Cooperative interactions among the other SDRCC Cores will occur with ease. Since this Core is a portion of Vanderbilt's Institutional Core system, liaisons are well established with other relevant Cores such as the Human Tissue and Pathology Shared Resource, Molecular Recognition (Antisera Production) Core, Small Animal Imaging Core, the Electron Microscopy Core, Proteomics Core or Cell Imaging/Confocal Core.